Olfactory ensheathing cells are attracted to, and can endocytose, bacteria
Olfactory ensheathing cells are attracted to, and can endocytose, bacteria
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DOI：
10.1007/s00018-008-8184-1
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发表时间：
2008-09-01
影响因子：
8
通讯作者：
Chuah, M. I.
中科院分区：
文献类型：
--
作者：
Leung, J. Y. K.;Chapman, J. A.;Chuah, M. I.
此前已证明嗅鞘细胞（OEC）表达Toll样受体，并通过将核因子-kappa B从细胞质转移到细胞核来对细菌做出反应。在这项研究中，我们表明，嗅鞘细胞延长显着更多的伪足时，他们暴露于大肠杆菌比在没有细菌（p=0.019）。免疫共沉淀结果表明，E. coli与OECs的结合是通过Toll样受体4介导的。Lyso-Tracker，一种选择性地在溶酶体中积累的荧光探针，以及对1型溶酶体相关膜蛋白的染色表明，内吞的FITC结合的E。大肠杆菌移位至溶酶体。它们似乎随后被分解，如透射电子显微镜所示。当OECs与惰性荧光微球孵育时，观察到没有明显的粘附膜和较少的吞噬作用。嗅鞘细胞内吞细菌的能力支持了嗅鞘细胞通过保护嗅觉组织免受细菌感染而发挥先天免疫功能的观点。
Olfactory ensheathing cells (OECs) have been shown previously to express Toll-like receptors and to respond to bacteria by translocating nuclear factor-kappa B from the cytoplasm to the nucleus. In this study, we show that OECs extended significantly more pseudopodia when they were exposed to Escherichia coli than in the absence of bacteria (p=0.019). Co-immunoprecipitation showed that E. coli binding to OECs was mediated by Toll-like receptor 4. Lyso-Tracker, a fluorescent probe that accumulates selectively in lysosomes, and staining for type 1 lysosome-associated membrane proteins demonstrated that endocytosed FITC-conjugated E. coli were translocated to lysosomes. They appeared to be subsequently broken down, as shown by transmission electron microscopy. No obvious adherence to the membrane and less phagocytosis was observed when OECs were incubated with inert fluorescent microspheres. The ability of OECs to endocytose bacteria supports the notion that OECs play an innate immune function by protecting olfactory tissues from bacterial infection.