Electroacupuncture improves cognitive deficits associated with AMPK activation in SAMP8 mice
Electroacupuncture improves cognitive deficits associated with AMPK activation in SAMP8 mice
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DOI：
10.1007/s11011-014-9641-1
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发表时间：
2015-06-01
影响因子：
3.6
通讯作者：
Shi, Hong
中科院分区：
文献类型：
--
作者：
Dong, Weiguo;Guo, Wanqing;Shi, Hong
脑能量代谢紊乱与阿尔茨海默病（AD）有关。腺苷一磷酸激活激酶（AMPK）是一种主要的能量传感器，可监测关键能量代谢物的水平。电针（EA）已被证明具有治疗AD的潜力。本研究观察了电针对快速老化小鼠模型SAMP 8（senescence-accelerated mouse prone 8，SAMP 8）认知功能的影响及AMPK及其磷酸化形式（p-AMPK）表达的变化。采用Morris水迷宫实验检测电针治疗后SAMP 8小鼠的认知功能。然后处死小鼠进行免疫组织化学和蛋白质印迹分析。电针可明显改善AD小鼠的记忆障碍，并提高海马中p-AMPK的表达。这些结果表明，电针改善认知功能与AMPK激活有关，AMPK可能是电针治疗AD的分子靶点。
Perturbations of brain energy metabolism are involved in Alzheimer's disease (AD). Adenosine monophosphate-activated kinase (AMPK) is a master energy sensor that monitors the levels of key energy metabolites. Electroacupuncture (EA) has demonstrated therapeutic potential for the treatment of AD. The effects of EA on cognitive functions and the changes of AMPK and its phosphorylated form (p-AMPK) expression were investigated in senescence-accelerated mouse prone 8 (SAMP8) mice. Cognitive function of SAMP8 mice was assessed using Morris water maze test after EA treatment. Then mice were sacrificed for immunohistochemistry and western blot analysis. EA stimulation significantly alleviated memory impairment of AD mice, and increased the levels of p-AMPK in the hippocampus. These results suggest that EA improved cognitive function associated with AMPK activation, AMPK may be a molecular target of EA in treating AD.