Comparing bulk extraction methods for chemically available polycyclic aromatic hydrocarbons with bioaccumulation in worms
Comparing bulk extraction methods for chemically available polycyclic aromatic hydrocarbons with bioaccumulation in worms
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DOI：
10.1071/en07017
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发表时间：
2007-01-01
影响因子：
4.3
通讯作者：
Pelletier, Emilien
中科院分区：
文献类型：
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作者：
Barthe, Mickael;Pelletier, Emilien
本研究的目的是评价不同的评估污染沉积物中多环芳烃(PAHs)有效性的技术。这一目标是通过比较沙蚕和海龙对多环芳烃的28天吸收实验结果与三种非穷尽提取方法提取的多环芳烃的结果来实现的：正丁醇(BuOH，100%)、羟丙基-β-环糊精(HPCD)水溶液和Brij700(B700)表面活性剂溶液。我们的结果强调了在尝试使用固/液提取方法预测多环芳烃在生物采样器(如蠕虫)中的生物积累时，同时考虑沉积物中的多环芳烃水平和多环芳烃的分子尺寸的重要性。表面活性剂B700溶液在暴露于未加添加剂的高污染沉积物(25-5700µg g(-1))时，可相当成功地预测多环芳烃的生物累积。当使用低污染沉积物(0.06-11mgg(-1))时，HPCD和BuOH是较好的萃取剂，而B700对于大多数样品来说似乎太温和了。我们的结果说明了寻找多环芳烃生物利用度的适当化学预测因子的兴趣和困难，特别是因为多环芳烃浓度和封存过程在结果质量中起着决定性的作用。由于B700不具有膨胀性，而且提取液易于制备，因此提出了一种使用该表面活性剂的提取程序，可作为老化高污染沉积物的可靠预测指标。
The purpose of this study is to evaluate different techniques for assessing the availability of polycyclic aromatic hydrocarbons (PAHs) in contaminated sediments. This goal was achieved by comparing results from 28-day uptake experiments by Nereis virens and Lumbriculus variegatus with PAHs extracted by three non-exhaustive extraction methods using: n-Butanol (BuOH, 100%), an aqueous solution of hydroxypropyl-beta-cyclodextrin (HPCD) and a surfactant solution of Brij700 (B700). Our results highlight the importance of considering both the PAH level in sediments and the molecular size of PAHs when attempting to predict their bioaccumulation in a biological sampler like worms using a solid/liquid extraction method. The surfactant B700 solution was quite successful to predict PAH bioaccumulation when exposed to unspiked highly contaminated sediments (25-5700 mu g g(-1)). When low contaminated sediments (0.06-11 mu g g(-1)) were used, HPCD and BuOH were better extractants for estimating bioaccumulation whereas B700 appeared to be too mild an extractant for most samples. Our results illustrate the interest and difficulties in finding an adequate chemical predictor for PAH bioavailabilty, particularly because PAH concentrations and sequestration processes play a determining role in the quality of results. Because B700 is not expansive and extraction solutions are easy to prepare, an extraction procedure involving this surfactant is proposed as a reliable predictor for aged highly contaminated sediments.