Enzymic analysis of endomyocardial biopsy specimens from patients with cardiomyopathies.
Enzymic analysis of endomyocardial biopsy specimens from patients with cardiomyopathies.
复制标题
心肌病患者心内膜心肌活检标本的酶分析。
作者：
T. Peters;G. Wells;C. Oakley;I. Brooksby;B. S. Jenkins;M. Webb;D. Coltart
心肌活检是从患有肥厚性或充血性心肌病的患者身上获得的。使用高度灵敏的测定程序估计心肌主要亚细胞细胞器的标记酶。结果与从左心室功能良好或较差的瓣膜性心脏病患者的组织中获得的结果进行了比较。肥厚型心肌病患者的左心室心肌组织显示酶活性基本正常水平。在充血性心肌病中，右心室组织显示线粒体酶水平降低，而乳酸脱氢酶水平升高。充血性心肌病患者的左心室组织显示线粒体和肌原纤维酶水平降低，但乳酸脱氢酶水平升高。充血性心肌病中肌原纤维 Ca++ 激活的 ATP 水平降低与继发于瓣膜病的左心室功能受损的患者相似。有人认为，线粒体功能缺陷是充血性心肌病的一个特征，乳酸脱氢酶水平升高反映了一种代偿反应。
Myocardial biopsies have been obtained from patients with hypertrophic or congestive cardiomyopathies. Marker enzymes for the principal subcellular organelles of the myocardium were estimated using highly sensitive assay procedures. The results were compared with those obtained in tissue from patients with valvular heart disease with good or poor left ventricular function. Left ventricular myocardial tissue from patients with hypertrophic cardiomyopathy showed essentially normal levels of enzymic activities. In congestive cardiomyopathy, right ventricular tissue showed reduced levels of mitochondrial enzymes with increased levels of lactate dehydrogenase. Left ventricular tissue from patients with congestive cardiomyopathy showed reduced levels of mitochondrial and myofibril enzymes but high levels of lactate dehydrogenase. The reduced levels of myofibril Ca++-activated ATP in congestive cardiomyopathy is similar to that found in patients with impaired left ventricular function secondary to valvular disease. It is suggested that defective mitochondrial function is a characteristic feature of congestive cardiomyopathy and that the increased levels of lactate dehydrogenase reflect a compensatory response.