7.87 eV postionization of peptides containing tryptophan or derivatized with fluorescein
7.87 eV postionization of peptides containing tryptophan or derivatized with fluorescein
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DOI：
10.1016/j.apsusc.2006.02.156
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发表时间：
2006-07-30
影响因子：
6.7
通讯作者：
Moore, J. F.
中科院分区：
文献类型：
--
作者：
Hanley, L.;Edirisinghe, P. D.;Moore, J. F.
蒽等化学标签可以附着在分子分析物上，并通过降低标记分子复合物的整体电离势来充当 7.87 eV 激光定位的发色团。荧光素和色氨酸被证明是两种新标签，可用于各种氨基酸和肽的 7.87 eV 激光定位。作为有效荧光探针的其他分子种类也应充当 7.87 eV 位置化的标签，因为它们显示出具有扩展 π 共轭的最高占据分子轨道，导致电离势低于该光子能量，并且能够稳定自由基阳离子的净正电荷。该技术在此针对激光解吸物质进行了演示，但也适用于 keV 离子溅射中性物质。总体而言，衍生物质的 7.87 eV 激光定位有望扩展质谱表面分析的能力。 (c) 2006 Elsevier B.V. 保留所有权利。
Chemical tags such as anthracene can be attached to a molecular analyte and serve as chromophores for 7.87 eV laser postionization by lowering the overall ionization potential of the tagged molecular complex. Fluorescein and tryptophan are demonstrated as two new tags for 7.87 eV laser postionization of various amino acids and peptides. Other molecular species that are efficient fluorescence probes should also serve as tags for 7.87 eV postionization since they display highest occupied molecular orbitals with extended pi-conjugation that lead to ionization potentials below this photon energy and an ability to stabilize the net positive charge of the radical cations. This technique is demonstrated here for laser desorbed species, but is also applicable to keV ion sputtered neutrals. Overall, 7.87 eV laser postionization of derivatized species promises to expand the capabilities of mass spectrometric surface analysis. (c) 2006 Elsevier B.V. All rights reserved.