INTERFERENCE REFLECTION MICROSCOPIC STUDY OF DORSAL-ROOT GROWTH CONES ON DIFFERENT SUBSTRATES - ASSESSMENT OF GROWTH CONE SUBSTRATE CONTACTS
INTERFERENCE REFLECTION MICROSCOPIC STUDY OF DORSAL-ROOT GROWTH CONES ON DIFFERENT SUBSTRATES - ASSESSMENT OF GROWTH CONE SUBSTRATE CONTACTS
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DOI：
10.1002/jnr.490210222
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发表时间：
1988-10-01
影响因子：
4.2
通讯作者：
GUNDERSEN, RW
中科院分区：
文献类型：
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作者：
GUNDERSEN, RW
用干涉反射显微镜观察了背根生长锥与IV型胶原、纤连蛋白、多聚赖氨酸和层粘连蛋白基质的接触情况，并观察了基质相关膜（SAM）。胶原蛋白和纤连蛋白的生长锥的X光图像表明基质和生长锥的基底区域之间的紧密关联的实质性暗区。薄的膜状面纱和片状伪足显得明亮，表明与基质的联系大大减少。丝状伪足可以是完全黑暗或光明，但通常是黑暗的穿插与明亮的地区，表明不同程度的接触。多聚赖氨酸和层粘连蛋白上的生长锥的IRM图像表明生长锥的基底区域与基质之间的联系减弱。面纱和板状足的外观与胶原和纤连蛋白上观察到的相似。SAM的观察表明，与层粘连蛋白和多聚赖氨酸相比，生长锥与胶原和纤连蛋白底物之间的接触程度增加。总的来说，这些数据表明，简单的增加粘附是不足以解释基板的偏好和增强神经突伸长。粘附力的整体下降可能会实现相同的现象，这表明，它可能是更重要的是考虑区域分布的粘合剂接触生长锥运动。
The contact between dorsal root growth cones and substrates of type IV collagen, fibronectin, poly-L-lysine, and laminin were studied using interference reflection microscopy (IRM) coupled with detaching growth cones from these substrates and observing substrate-associated membrane (SAM). IRM images of growth cones of collagen and fibronectin indicate substantial dark areas of close association between the substrate and basal region of the growth cone. The thin membraneous veils and lamellapodia appear bright, indicating a greatly decreased association with the substrate. Filopodia can appear either entirely dark or light but usually are dark interspersed with brighter areas indicating varying degrees of contact. IRM images of growth cones on polylysine and laminin suggested a decreased association between the basal region of the growth cone and the substrate. The appearance of veils and lamellapodia was similar to that observed on collagen and fibronectin. Observations of SAM indicate an increased degree of contact between growth cones and substrates of collagen and fibronectin compared to laminin and polylysine. Collectively these data indicate that simple increases in adhesion are not adequate to explain substrate preference and enhancement of neurite elongation. Overall decreases in adhesion may accomplish the same phenomena, suggesting that it is perhaps more important to consider the regional distribution of adhesive contacts in relation to growth cone movement.