Cell nuclei have lower refractive index and mass density than cytoplasm
Cell nuclei have lower refractive index and mass density than cytoplasm
复制标题
细胞核的折射率和质量密度比细胞质低。
DOI：
10.1002/jbio.201500273
复制
发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Chan, Chii J.
中科院分区：
文献类型：
--
作者：
Schuermann, Mirjam;Scholze, Jana;Chan, Chii J.
人们普遍认为细胞核是一个密集的物体，具有比周围细胞质更高的折射率 (RI) 和质量密度。在这里，分离的细胞核的体积会因静电和渗透条件以及改变染色质构象的药物处理而系统地变化。孤立核的折射率和干质量源自使用数字全息显微镜 (DHM) 进行的定量相位测量。令人惊讶的是，在四种不同的细胞系中以及整个细胞周期中，发现细胞核的 RI 和质量密度低于细胞质。这一结果对于概念化光组织相互作用以及细胞中的生物过程具有重要意义。
Common perception regards the nucleus as a densely packed object with higher refractive index (RI) and mass density than the surrounding cytoplasm. Here, the volume of isolated nuclei is systematically varied by electrostatic and osmotic conditions as well as drug treatments that modify chromatin conformation. The refractive index and dry mass of isolated nuclei is derived from quantitative phase measurements using digital holographic microscopy (DHM). Surprisingly, the cell nucleus is found to have a lower RI and mass density than the cytoplasm in four different cell lines and throughout the cell cycle. This result has important implications for conceptualizing light tissue interactions as well as biological processes in cells.