Sex difference and steroid modulation of pheromone-induced immediate early genes in the two zones of the mouse accessory olfactory system
Sex difference and steroid modulation of pheromone-induced immediate early genes in the two zones of the mouse accessory olfactory system
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DOI：
10.1523/jneurosci.21-07-02474.2001
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发表时间：
2001-04-01
影响因子：
5.3
通讯作者：
Cherry, JA
中科院分区：
文献类型：
--
作者：
Halem, HA;Baum, MJ;Cherry, JA
犁鼻器（VNO）和副嗅球（AOB）内的两个解剖学和神经化学上不同的区域已被确定为负责检测信息素。使用标记来区分VNO神经上皮细胞的顶端和基底神经元和头侧与尾侧AOB肾小球，我们研究了立即早期基因免疫反应性（IEG-IR）在性腺切除，类固醇治疗的小鼠响应于信息素的男性和女性同种。暴露后，雌二醇处理的女性弄脏男性床上用品，更多的VNO神经元在基底层比顶端表现出IEG-IR相比，雌二醇处理的男性VNO神经元。相反，而污染的女性床上用品未能诱导IEG-IR在VNO神经元的雌二醇治疗的男性或女性，顶端和基底神经元被激活睾酮治疗的男性。男性和女性信息素也激活二尖瓣和颗粒细胞的AOBs的所有科目，但不同的信息素的反应分布在整个边界的吻和尾区域。这些数据表明，男性和女性的反应相同的信息素刺激的差异被发现在感觉神经元的VNO。我们建议离心。VNO神经元的去甲肾上腺素能输入。其在两性中可能不同并且对成人性类固醇的反应不同，调节对信息素刺激的敏感性。
Two anatomically and neurochemically distinct zones within the vomeronasal organ (VNO) and accessory olfactory bulb (AOB) have been identified that are responsible for the detection of pheromones. Using markers to distinguish between apical and basal neurons of the VNO neuroepithelium and rostral versus caudal AOB glomeruli, we examined immediate early gene immunoreactivity (IEG-IR) in gonadectomized, steroid-treated mice in response to pheromones of male and female conspecifics. After exposure of estradiol-treated females to soiled male bedding, more VNO neurons in the basal than the apical layer exhibited IEG-IR compared with VNO neurons of estradiol-treated males. Conversely, whereas soiled female bedding failed to induce IEG-IR in VNO neurons of estradiol-treated males or females, both apical and basal neurons were activated in testosterone-treated males. Male and female pheromones also activated mitral and granule cells in the AOBs of all subjects, but responses to different pheromones were distributed across the boundary of the rostral and caudal regions. These data show that differences in the response of males and females to the same pheromonal stimulus are found in the sensory neurons of the VNO. We propose that centrifugal. noradrenergic inputs to VNO neurons. which may differ in the two sexes and respond differently to adult sex steroids, modulate sensitivity to pheromonal stimulation.