INTIMAL LESION FORMATION IN RAT CAROTID ARTERIES AFTER ENDOTHELIAL DENUDATION IN ABSENCE OF MEDIAL INJURY
INTIMAL LESION FORMATION IN RAT CAROTID ARTERIES AFTER ENDOTHELIAL DENUDATION IN ABSENCE OF MEDIAL INJURY
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DOI：
10.1161/01.atv.10.6.1082
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发表时间：
1990-11-01
期刊：
影响因子：
--
通讯作者：
REIDY, MA
中科院分区：
文献类型：
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作者：
FINGERLE, J;AU, YPT;REIDY, MA
球囊导管通过造成的动脉损伤会导致内皮剥脱和内侧损伤，并产生明显的平滑肌细胞 (SMC) 增殖反应。在这项研究中，通过使用 5/0 单丝缝合线的旋转环（温和剥脱技术）去除大鼠颈动脉的内皮，这不会对下面的内侧细胞造成任何可检测到的损伤，但确实会导致血小板粘附。血小板源性生长因子 (PDGF) A 链和 PDGF 受体 mRNA 的表达与气球状颈动脉中的表达相当，但与观察到的气球状剥脱相比，内侧 SMC 对轻微剥脱的增殖反应显着降低（1.4% 对 13.6%）。仅在裸露超过 7 天的区域观察到内膜病变。这些结果表明，没有内侧创伤的剥脱性损伤足以诱发内膜病变，并且在气球状血管中观察到的显着较高的增殖可能反映了内侧细胞对剥脱期间发生的创伤的反应。
Injury of an artery by passage of a balloon catheter causes both endothelial denudation and medial damage and produces a marked smooth muscle cell (SMC) proliferative response. In this study, the endothelium from rat carotid arteries was removed by use of a rotating loop of 5/0 monofilament suture (gentle denudation technique), which did not cause any detectable damage to the underlying medial cells but did cause platelet adherence. Expression of platelet-derived growth factor (PDGF) A-chain and PDGF receptor mRNA was comparable to that seen in ballooned carotids, but the medial SMC proliferative response to gentle denudation was markedly reduced when compared to that observed that balloon denudation (1.4% vs. 13.6%). Intimal lesions were only observed in those zones that remained denuded for more than 7 days. These results demonstrate that a denuding injury with no medial trauma is sufficient to induce intimal lesions and that the significantly higher proliferation seen in ballooned vessels might reflect a response of the medial cells to trauma that occurred during denudation.