PROTECTIVE EFFECTS OF TOWNE CYTOMEGALO-VIRUS VACCINE AGAINST LOW-PASSAGE CYTOMEGALO-VIRUS ADMINISTERED AS A CHALLENGE
PROTECTIVE EFFECTS OF TOWNE CYTOMEGALO-VIRUS VACCINE AGAINST LOW-PASSAGE CYTOMEGALO-VIRUS ADMINISTERED AS A CHALLENGE
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DOI：
10.1093/infdis/159.5.860
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发表时间：
1989-05-01
影响因子：
6.4
通讯作者：
WEIBEL, RE
中科院分区：
文献类型：
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作者：
PLOTKIN, SA;STARR, SE;WEIBEL, RE
为了测试Towne减毒人类巨细胞病毒（HCMV）活疫苗对正常人的保护作用，我们开发了一种由低传代分离株（托莱多菌株）组成的肠外攻击，该菌株按分级剂量皮下接种。在剂量为10或100 pfu的血清阴性个体中，这种攻击病毒引起轻度单核细胞增多症。该病伴有非典型淋巴细胞增多、肝酶升高、HCMV排泄和HCMV特异性免疫反应。自然血清阳性的志愿者在接受1000 pfu的托莱多刺激后也出现了临床和实验室感染证据，但抵抗10或100 pfu。早些时候接种过疫苗的志愿者也对托莱多10或100 pfu引起的疾病有抵抗力，尽管有些人被100 pfu剂量感染后无症状。当托莱多攻毒剂量为10 pfu时，疫苗诱导的HCMV免疫与自然诱导的免疫一样完全。
To test the protective effect of Towne live attenuated human cytomegalovirus (HCMV) vaccine in normal individuals, we developed a parenteral challenge consisting of a low passage isolate (Toledo strain) inoculated subcutaneously in graded doses. This challenge virus caused a mild mononucleosis syndrome in seronegative individuals at doses of 10 or 100 pfu. The illness was accompanied by atypical lymphocytosis, raised hepatic enzymes, excretion of HCMV and HCMV-specific immune responses. Naturally seropositive volunteers also developed clinical and laboratory evidence of infection after challenge with 1,000 pfu of Toledo but resisted 10 or 100 pfu. Volunteers who had been vaccinated 1 y earlier also were resistant to disease caused by 10 or 100 pfu of Toledo, although some were asymptomatically infected by the 100 pfu dose. Vaccine-induced immunity to HCMV was as complete as naturally induced immunity when the challenge dose of Toledo was 10 pfu.