Retinal detachment model in rodents by subretinal injection of sodium hyaluronate.
Retinal detachment model in rodents by subretinal injection of sodium hyaluronate.
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DOI：
10.3791/50660
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发表时间：
2013-09-11
期刊：
影响因子：
--
通讯作者：
Vavvas DG
中科院分区：
文献类型：
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作者：
Matsumoto H;Miller JW;Vavvas DG
视网膜下注射透明质酸钠是一种被广泛接受的诱导视网膜脱离（RD）的方法。然而，RD的高度和持续时间或视网膜下出血的发生可影响视网膜脱离中的感光细胞死亡。因此，它是有利的，以创建可再现的RD没有视网膜下出血的评估感光细胞死亡。我们修改了以前报道的方法，在可重复的位置创建大泡性和持续性RD，很少发生视网膜下出血。这种改良方法的关键步骤是创建一个自密封巩膜切口，它可以防止透明质酸钠注入视网膜下腔后泄漏。为了形成自密封巩膜切口，创建巩膜隧道，然后用30 G针将巩膜穿透到脉络膜中。虽然脉络膜出血可能发生在这一步骤中，收敛与外科矛减少脉络膜出血的发生率。该方法允许在涉及RD的疾病中的感光细胞死亡的更可再现和可靠的模型，所述疾病例如孔源性RD、早产儿视网膜病变、糖尿病视网膜病变、中心性浆液性脉络膜视网膜病变和年龄相关性黄斑变性（AMD）。
Subretinal injection of sodium hyaluronate is a widely accepted method of inducing retinal detachment (RD). However, the height and duration of RD or the occurrence of subretinal hemorrhage can affect photoreceptor cell death in the detached retina. Hence, it is advantageous to create reproducible RDs without subretinal hemorrhage for evaluating photoreceptor cell death. We modified a previously reported method to create bullous and persistent RDs in a reproducible location with rare occurrence of subretinal hemorrhage. The critical step of this modified method is the creation of a self-sealing scleral incision, which can prevent leakage of sodium hyaluronate after injection into the subretinal space. To make the self-sealing scleral incision, a scleral tunnel is created, followed by scleral penetration into the choroid with a 30 G needle. Although choroidal hemorrhage may occur during this step, astriction with a surgical spear reduces the rate of choroidal hemorrhage. This method allows a more reproducible and reliable model of photoreceptor death in diseases that involve RD such as rhegmatogenous RD, retinopathy of prematurity, diabetic retinopathy, central serous chorioretinopathy, and age-related macular degeneration (AMD).