Open Surface Droplet Microfluidic Magnetosensor for Microcystin-LR Monitoring in Reservoir
Open Surface Droplet Microfluidic Magnetosensor for Microcystin-LR Monitoring in Reservoir
复制标题
用于水库中微囊藻毒素-LR 监测的开放式表面液滴微流体磁传感器。
DOI：
10.1021/acs.analchem.9b05516
复制
发表时间：
2020-02-18
影响因子：
7.4
通讯作者：
Lei, Hongtao
中科院分区：
文献类型：
--
作者：
Guan, Tian;He, Jianfei;Lei, Hongtao
Establishing rapid, simple, and in situ detection of rnicrocystin-LR (MC-LR) in drinking water sources is of significant importance for human health. To ease the situation that current methods cannot address, an open surface droplet microfluidic magnetosensor was designed and validated to quantify MC-LR in reservoir water, which is capable of (1) MC-LR isolation via MC-LR antibody-conjugated magnetic beads, (2) parallel and multistep analytical procedures in 15-array power-free and reusable active droplet microfluidic chips, (3) immunoassay incubation and fluorescence excitation within a miniaturized multifunctional 3D-printing optosensing accessory, and (4) signal read-out and data analysis by a user-friendly Android app. The proposed smartphone-based fluorimetric magnetosensor exhibited a low limit of detection of 1.2 x 10(-5) mu g/L in the range of 10(-4) mu g/L to 100 mu g/L. This integrated and high throughput platform was utilized to draw an MC-LR contamination map for six reservoirs distributed in the Pearl River delta, Guangdong Province. It promises to be a simple and successful quantification method for MC-LR field detection, bringing many benefits to rapid on-site screening.
