BLUE-LIGHT REGULATES THE ACCUMULATION OF 2 PSBD-PSBC TRANSCRIPTS IN BARLEY CHLOROPLASTS
BLUE-LIGHT REGULATES THE ACCUMULATION OF 2 PSBD-PSBC TRANSCRIPTS IN BARLEY CHLOROPLASTS
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DOI：
10.1002/j.1460-2075.1989.tb08424.x
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发表时间：
1989-10-01
期刊：
影响因子：
11.4
通讯作者：
MULLET, JE
中科院分区：
文献类型：
--
作者：
GAMBLE, PE;MULLET, JE
D2（一种由 psbD 编码的光系统 II 反应中心蛋白）的合成在光诱导叶绿体成熟过程中得到差异性维持。 D2 的持续合成与两个 psbD-psbC 转录物的选择性光诱导积累同时进行，这两个转录物具有共同的 5'' 末端。在本研究中，我们研究了光感受器的性质以及 psbD-psbC 转录诱导的注量要求。将 4.5 天大的黑暗生长的大麦幼苗转移到光下后 1 至 2 小时内，可以检测到光诱导的 psbD-psbC RNA 群体变化。光诱导的转录物积累通常发生在叶绿素缺陷大麦突变体 xan-f10 中，表明光激活的叶绿素形成和光合作用对于 RNA 诱导来说不是必需的。高通量蓝光充分诱导psbD-psbC转录物积累；低或高通量的红光或远红光则不然。然而，如果在蓝光处理后立即给予远红光，则蓝光脉冲引起的 psbD-psbC 转录物积累可能会部分减弱。因此，虽然需要蓝光来启动转录物积累，但光敏色素调节反应的幅度。用放线菌酮预处理黑暗生长的植物可阻止光诱导的 psbC-psbC 转录物积累。这可能表明蓝光响应核基因参与了光诱导的两个 psbD-psbC 转录物的积累。
Synthesis of D2, a Photosystem II reaction center protein encoded by psbD, is differentially maintained during light-induced chloroplast maturation. The continued synthesis of D2 is paralleled by selective light-induced accumulation of two psbD-psbC transcripts which share a common 5'' terminus. In the present study, we examine the nature of the photoreceptor and the fluence requirement for psbD-psbC transcript induction. The light-induced change in psbD-psbC RNA population can be detected between 1 and 2 h after 4.5 day old dark-grown barley seedlings are transferred to the light. Light-induced transcript accumulation occurs normally in the chlorophyll-deficient barley mutant, xan-f10, indicating that light-activated chlorophyll formation and photosynthesis are not required for RNA induction. High fluence blue light fully induces psbD-psbC transcript accumulation; low or high fluence red or far-red light do not. However, psbD-psbC transcript accumulation elicited by blue light pulses can be partially attenuated if far-red light is given immediately following the blue light treatment. Thus, although blue light is needed to initiate transcript accumulation, phytochrome modulates the amplitude of the response. Pretreatment of dark-grown plants with cycloheximide blocks light-induced psbC-psbC transcript accumulation. This could implicate a blue-light responsive nuclear gene in the light-induced accumulation of the two psbD-psbC transcripts.