Immunohistochemical analysis of bcl-2 protein expression in renal cell carcinoma
Immunohistochemical analysis of bcl-2 protein expression in renal cell carcinoma
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DOI：
10.1016/s0022-5347(05)68635-1
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发表时间：
1999-08-01
影响因子：
6.6
通讯作者：
Low, RK
中科院分区：
文献类型：
--
作者：
Huang, A;Fone, PD;Low, RK
目的：探讨bcl2蛋白在人肾细胞癌中的表达情况、范围和分布。材料与方法：采用免疫组织化学染色技术，对因肾细胞癌或创伤切除的肾癌标本及3种肾癌细胞系中bcl2蛋白的表达进行分析。在组织学正常对照组和肿瘤附近区域，仅观察到被认为是肾细胞癌起源的近端肾小管细胞的罕见和少量染色。结论：bcl2是一种已知的调节细胞凋亡(细胞程序性死亡)的原癌基因。BCL-2蛋白在大多数被检测的肾癌中过表达。BCL-2过表达可能在肿瘤发生中起作用，并可能解释肾癌对化疗药物和放射治疗的相对耐药。
Purpose: To investigate the incidence, extent, and distribution of bcl-2 protein expression in human renal cell carcinomas.Materials and Methods: Using immunohistochemical tissue staining techniques, bcl-2 protein expression was analyzed in archival nephrectomy specimens removed for renal cell carcinoma or trauma and in 3 renal cell carcinoma cell lines.Results: Normal kidneys demonstrated bcl-2 immunopositivity primarily within the cytoplasm of distal tubule cells. Only rare and minor staining of the proximal tubular cells, thought to be the origin of renal cell carcinoma, was noted in histologically normal controls and areas adjacent to tumor. In contrast, bcl-2 protein expression was demonstrated in 70% of renal cell carcinomas and in all 3 experimental cell lines.Conclusions: Bcl-2 is a proto-oncogene known to regulate apoptosis (programmed cell death). Bcl-2 protein is overexpressed in the majority of renal cell carcinomas examined. Bcl-2 overexpression may have a role in tumorigenesis and may explain the relative resistance of renal cell carcinoma to chemotherapeutic agents and to radiation therapy.