[Determination of plasma protein binding rates of nine compounds of Inula cappa extraction based on method of equilibrium dialysis].
[Determination of plasma protein binding rates of nine compounds of Inula cappa extraction based on method of equilibrium dialysis].
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DOI：
10.19540/j.cnki.cjcmm.20190116.007
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发表时间：
2019-04-01
期刊：
影响因子：
--
通讯作者：
Gong, Zi-Peng
中科院分区：
文献类型：
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作者：
Bao, Hong-Song;Hou, Jing-Yu;Gong, Zi-Peng
用平衡透析法测定牛蒡提取物中9种化合物的血浆蛋白结合率。用甲醇沉淀血浆样品中的蛋白质，建立超高效液相色谱-串联质谱法（UPLC-MS/MS），以葛根素为内标，测定绿原酸、东莨菪碱、新绿原酸、隐绿原酸、1,3- o -二咖酰奎宁酸、没食草素、3,4- o -二咖酰奎宁酸、3,5- o -二咖酰奎宁酸、4,5- o -二咖酰奎宁酸等9种活性物质的浓度。各成分线性关系良好（r≥0.999），准确度、精密度、提取回收率和稳定性符合测定要求，最佳浓度范围内无内源化合物干扰。结果表明，该方法稳定可靠，可用于大鼠和人血浆中九种活性化合物的血浆蛋白结合率的测定。9种活性化合物在大鼠和人血浆中的蛋白结合率分别为（41.07±0.046）% ~(94.95±0.008)%和（37.66±0.043）% ~(97.46±0.013)%。结果表明，牛蒡子提取物中9种化合物的血浆蛋白结合率在大鼠和人之间存在差异。
To determine the plasma protein binding rate of the nine compounds in Inula cappa extraction by the method of equilibrium dialysis. The proteins in plasma samples were precipitated by methanol, and the ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for determination of the concentrations of the nine active compounds, namely chlorogenic acid, scopolin, neochlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, galuteolin, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, with the internal standard of puerarin. We found that all components have a good linearity(r≥0.999), and accuracy, precision, extraction recovery and stability conformed to the requirements of determination, without endogenous compounds disturbing within the range of optimum concentration. This suggested that the method was stable and reliable, and could be used for the determination of the plasma protein binding rates of the nine active compounds in rat and human plasma of I. cappa. The plasma protein binding rates of the nine active compounds in rat and human plasma respectively were(41.07±0.046)%-(94.95±0.008)%, and(37.66±0.043)%-(97.46±0.013)%. According to the results, there were differences in the plasma protein binding rates of the nine compounds in I. cappa extraction between rat and human.