Characterization of a human hematopoietic progenitor cell capable of forming blast cell containing colonies in vitro.
Characterization of a human hematopoietic progenitor cell capable of forming blast cell containing colonies in vitro.
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能够在体外形成含有母细胞集落的人类造血祖细胞的表征。
DOI：
10.1172/jci113658
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hoffman,R
中科院分区：
文献类型：
--
作者：
Brandt,J;Baird,N;Lu,L;Srour,E;Hoffman,R
利用半固体培养系统，检测了一种能够在体外产生含菌落的胚细胞的造血细胞（CFU-B1）。CFU-B1具有自我更新的能力，并致力于许多造血谱系。采用人祖细胞抗原-1 （human祖细胞antigen-1， HPCA-1）单克隆抗体和主要组织相容性ⅱ类抗原（major tissue compatibility class II antigen, HLA-DR）单克隆抗体进行荧光活化细胞分选，对CFU-B1进行表型分析。发现CFU-B1表达My10，但不表达HLA-DR抗原；利用补体依赖性细胞毒性消除DR阳性细胞的实验证实了这一发现。用5-氟尿嘧啶和4-氢过氧环磷酰胺两种化疗药物预处理骨髓细胞有助于CFU-B1衍生集落的检测，同时减少或完全抑制其他造血祖细胞的集落形成。cfu - b1衍生的集落形成依赖于外源性造血生长因子的添加。由人膀胱癌细胞系5637或凝集素刺激的白细胞，以及重组粒细胞-巨噬细胞集落刺激因子、白细胞介素3或白细胞介素1 α调节的培养基促进了母细胞集落的形成。相比之下，重组红细胞生成素、重组白细胞介素4、纯化的巨噬细胞集落刺激因子或重组粒细胞集落刺激因子单独都不能促进母细胞集落的形成。图片
A hematopoietic cell (CFU-B1) capable of producing blast cell containing colonies in vitro was detected using a semisolid culture system. The CFU-B1 has the capacity for self-renewal and commitment to a number of hematopoietic lineages. Monoclonal antibody to the human progenitor cell antigen-1 (HPCA-1) and a monoclonal antibody against the major histocompatibility class II antigen (HLA-DR) were used with fluorescence activated cell sorting to phenotype the CFU-B1. The CFU-B1 was found to express My10 but not HLA-DR antigen; experiments using complement-dependent cytotoxicity to eliminate DR positive cells confirmed this finding. Pretreatment of marrow cells with two chemotherapeutic agents, 5-fluorouracil and 4-hydroperoxycyclophosphamide facilitated detection of CFU-B1 derived colonies, while diminishing or totally inhibiting colony formation by other hematopoietic progenitor cells. CFU-B1-derived colony formation was dependent upon the addition of exogenous hematopoietic growth factors. Media conditioned either by the human bladder carcinoma cell line 5637 or lectin stimulated leukocytes, as well as recombinant granulocyte-macrophage colony stimulating factor, interleukin 3 or interleukin 1 alpha promoted blast cell colony formation. By contrast, neither recombinant erythropoietin, recombinant interleukin 4, purified macrophage colony stimulating factor or recombinant granulocyte colony-stimulating factor alone promoted blast cell colony formation.Images