Distribution of AChE in cholinergic and non-cholinergic neurons
Distribution of AChE in cholinergic and non-cholinergic neurons
复制标题
AChE 在胆碱能和非胆碱能神经元中的分布
DOI：
10.1016/0006-8993(81)90281-x
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
B. G. Wallace
中科院分区：
文献类型：
--
作者：
B. G. Wallace
细胞内和表面乙酰胆碱酯酶活性测定的个别胆碱能和非胆碱能神经元解剖水蛭的中枢神经系统。采用乙硫氨酸预处理选择性抑制胞外酶。释放乙酰胆碱作为递质的细胞内乙酰胆碱酯酶活性水平高出约10倍，而所有神经元与细胞表面相关的活性水平相似。这些结果表明，细胞内胆碱酯酶可能是一个有用的标记胆碱能神经元。
Intracellular and surface acetylcholinesterase activities were determined for individual cholinergic and non-cholinergic neurons dissected from the central nervous system of the leech. Echothiophate pretreatment was used to inhibit selectively extracellular enzyme. Cells releasing acetylcholine as a transmitter had approximately 10-fold higher levels of intracellular acetylcholinesterase activity, while all neurons had similar levels of activity associated with the cell surface. These results suggest that intracellular cholinesterase may be a useful marker for cholinergic neurons.