Complete protein sequences of the variable regions of the cloned heavy and light chains of a human anti-cytomegalovirus antibody reveal a striking similarity to human monoclonal rheumatoid factors of the Wa idiotypic family.
Complete protein sequences of the variable regions of the cloned heavy and light chains of a human anti-cytomegalovirus antibody reveal a striking similarity to human monoclonal rheumatoid factors of the Wa idiotypic family.
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人抗巨细胞病毒抗体的克隆重链和轻链可变区的完整蛋白质序列揭示了与Wa独特型家族的人单克隆类风湿因子惊人的相似性。
DOI：
10.1172/jci113483
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Wasserman,RL
中科院分区：
文献类型：
--
作者：
Newkirk,MM;Gram,H;Heinrich,GF;Ostberg,L;Capra,JD;Wasserman,RL
人中和IgGl抗巨细胞病毒（CMV）抗体的重链和轻链多肽的可变区的完整氨基酸和核苷酸序列揭示了与Wa独特型家族的IgM类风湿因子（RF）的惊人同源性。抗CMV抗体和Wa RF具有共同的VKIIIb、JK1和VHIa基因区段，但使用不同的DH和JH基因区段。抗CMV抗体不具有RF活性且不表达Wa独特型。Wa RF不具有抗CMV活性。然而，Wa RF的一个子集和抗CMV抗体确实在VHIa和VKIIIb多肽上共享几种独特型。由于在抗原结合特征和其他一些表达的独特型方面存在重大差异，因此这些数据表明D和J区氨基酸对此类特异性至关重要。尽管在不同的免疫应答中使用这种高度同源的基因片段在鼠系统中有充分的记载，但这些数据代表了人类中的第一个这样的例子。
The complete amino acid and nucleotide sequences of the variable regions of the heavy and light polypeptide chains of a human neutralizing IgGl anti-cytomegalovirus (CMV) antibody reveal a striking homology to IgM rheumatoid factors (RFs) of the Wa idiotypic family. The anti-CMV antibody and Wa RFs have in common VKIIIb, JKl, and VHIa gene segments but use different DH and JH gene segments. The anti-CMV antibody does not have RF activity and does not express the Wa idiotype. The Wa RFs do not have anti-CMV activity. A subset of Wa RFs, however, and the anti-CMV antibody do share several idiotypes on the VHIa and VKIIIb polypeptides. Since there are major differences in the antigen binding characteristics and some of the other expressed idiotypes, these data suggest that the D and J region amino acids are crucial to such specificities. Although the use of such highly homologous gene segments in different immune responses is well-documented in murine systems, these data represent the first such example in the human.