Cronobacter gen nov, a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen nov, comb nov, Cronobacter malonaticus sp nov, Cronobacter turicensis sp nov, Cronobacter muytjensii sp nov, Cronobacter dublinensis sp nov, Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp dublinensis subsp nov, Cronobacter dublinensis subsp lausannensis subsp nov and Cronobacter dublinensis subsp lactaridi subsp nov
Cronobacter gen nov, a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen nov, comb nov, Cronobacter malonaticus sp nov, Cronobacter turicensis sp nov, Cronobacter muytjensii sp nov, Cronobacter dublinensis sp nov, Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp dublinensis subsp nov, Cronobacter dublinensis subsp lausannensis subsp nov and Cronobacter dublinensis subsp lactaridi subsp nov
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DOI：
10.1099/ijs.0.65577-0
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发表时间：
2008-06-01
影响因子：
2.8
通讯作者：
Joosten, Han
中科院分区：
文献类型：
--
作者：
Iversen, Carol;Mullane, Niall;Joosten, Han
阪崎肠杆菌是一种机会致病菌，可引起新生儿感染。本研究进一步阐明了被描述为[E] sakazakii的分离株的分类，并完成了将这些生物重新分类为新属（Cronobacter gen. 11 . [E] sakazakii）中的新种和亚种的正式描述。[E] sakazakii于1980年首次被定义，然而最近的多相分类分析已经确定该生物群由几个基因组种组成。在这项研究中，使用Biotype 100和Biolog表型微阵列数据扩展了提出的新物种的表型描述。进一步的DNA-DNA杂交实验表明，在sakazakii基因组种中，丙二酸阳性菌株代表一个独特的种，而不是一个亚种。DNA-DNA杂交还确定，在提出的物种（都柏林克罗诺杆菌sp. nov.）中，表型上不同的菌株属于同一物种，可以被认为是新的亚种。基于这些分析，提出了以下可选分类：坂崎克罗诺杆菌（Cronobacter sakazakii gen. nov., comb.）。11 .[型应变ATCC 29544(T) (=NCTC 11467(T))]；克罗诺杆菌[型株CDC 1058-77(T) (=LMG 23826(T) =DSM 18702(T))]；克罗诺杆菌[型株z3032(T) (=LMG 23827(T) =DSM 18703(T))]；克罗诺杆菌[型菌株ATCC 51329(T) (=Clp 103581(T))]；都柏林克罗诺杆菌[类型菌株DES187(T) (=LMG 23823(T) =DSM 18705(T))]；都柏林克罗诺杆菌亚种dublinensis无性系种群。11 .[型应变DES187(T) (=LMG 23823(T) =DSM 18705(T))]；都柏林克罗诺杆菌亚种lausannensis无性系种群。11 .[型菌株E515(T) (= LMG 23824=DSM 18706(T))]，都柏林克罗诺杆菌亚种；lactaridi无性系种群。11 .[型应变E464(T) (=LMG 23825(T)=DSM 18707(T))]。
[Enterobacter] sakazakii is an opportunistic pathogen that can cause infections in neonates. This study further clarifies the taxonomy of isolates described as [E] sakazakii and completes the formal description of the proposed reclassification of these organisms as novel species and subspecies within a proposed novel genus, Cronobacter gen. nov. [E] sakazakii was first defined in 1980, however recent polyphasic taxonomic analysis has determined that this group of organisms consists of several genomospecies. In this study, the phenotypic descriptions of the proposed novel species are expanded using Biotype 100 and Biolog Phenotype MicroArray data. Further DNA-DNA hybridization experiments showed that malonate-positive strains within the [E] sakazakii genomospecies represent a distinct species, not a subspecies. DNA-DNA hybridizations also determined that phenotypically different strains within the proposed species, Cronobacter dublinensis sp. nov., belong to the same species and can be considered as novel subspecies. Based on these analyses, the following alternative classifications are proposed: Cronobacter sakazakii gen. nov., comb. nov. [type strain ATCC 29544(T) (=NCTC 11467(T))]; Cronobacter malonaticus sp. nov. [type strain CDC 1058-77(T) (=LMG 23826(T) =DSM 18702(T))] Cronobacter turicensis sp. nov. [type strain z3032(T) (=LMG 23827(T) =DSM 18703(T))]; Cronobacter muytjensii sp. nov. [type strain ATCC 51329(T) (=Clp 103581(T))]; Cronobacter dublinensis sp. nov. [type strain DES187(T) (=LMG 23823(T) =DSM 18705(T))]; Cronobacter dublinensis subsp. dublinensis subsp. nov. [type strain DES187(T) (=LMG 23823(T) =DSM 18705(T))]; Cronobacter dublinensis subsp. lausannensis subsp. nov. [type strain E515(T) (= LMG 23824=DSM 18706(T))], and Cronobacter dublinensis subsp. lactaridi subsp. nov. [type strain E464(T) (=LMG 23825(T)=DSM 18707(T))].