DHA Protects Against Zinc Mediated Alterations in Neuronal Cellular Bioenergetics
DHA Protects Against Zinc Mediated Alterations in Neuronal Cellular Bioenergetics
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DOI：
10.1159/000331724
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Suphioglu, Cenk
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文献类型：
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作者：
McGee, Sean L.;Sadli, Nadia;Suphioglu, Cenk
锌蓄积可能损害细胞生物能量学，这与神经元凋亡有关。我们同时评估了活细胞中的无氧和有氧代谢，以证实这种效应，并假设omega 3脂肪酸二十二碳六烯酸（DHA）可以防止任何锌介导的生物能量学改变。在这项研究中，我们观察到细胞耗氧量的减少，但不是糖酵解速率，慢性锌暴露后，这是特定的神经细胞。这是由于受损的ATP周转，对线粒体功能没有任何其他影响，并通过DHA恢复。DHA对生物能量学没有进一步的影响。这些数据表明，锌破坏了呼吸链远端的生物能量学，而呼吸链是由DHA恢复的。版权所有（C）2011 S. Karger AG，巴塞尔
Zinc accumulation may impair cellular bioenergetics, which is associated with neuronal apoptosis. We simultaneously assessed anaerobic and aerobic metabolism in live cells to characterise this effect and hypothesised that the omega 3 fatty acid docosahexaenoic acid (DHA) would protect against any zinc mediated alterations in bioenergetics. In this study we observed a decrease in cellular oxygen consumption, but not glycolytic rate, following chronic zinc exposure, which was specific for neuronal cells. This was due to impaired ATP turnover, without any other effects on mitochondrial function, and was restored by DHA. DHA had no further effects on bioenergetics. These data suggest that zinc disrupts bioenergetics at a point distal to the respiratory chain, which is restored by DHA. Copyright (C) 2011 S. Karger AG, Basel