Factors influencing expression of mammary ductal dysplasia in cell dissociation-derived murine mammary outgrowths.
Factors influencing expression of mammary ductal dysplasia in cell dissociation-derived murine mammary outgrowths.
复制标题
影响细胞解离衍生的小鼠乳腺增生中乳腺导管发育不良表达的因素。
作者：
S. Ethier;R. Ullrich
乳腺导管发育不良的表达已被证明在源自酶解乳腺细胞的乳腺增生中增强，并受到用于衍生增生的细胞数量的影响。本研究旨在进一步检查这种细胞剂量效应，并确定生长物的发育状态或致癌剂施用和细胞解离之间的时间是否影响导管发育不良的表达和持续性。通过将 10(4) 或 10(5) 酶解乳腺细胞注射到 3 周龄雌性 BALB/c 小鼠的无腺体脂肪垫中，获得乳腺增生。供体动物未经治疗或暴露于7,12-二甲基苯(a)蒽或伽马射线照射。根据实验，在移植后 4.5、8、10 或 16 周检查生长物，并将其分类为正常或发育不良生长。数据表明，无论衍生物的发育状态如何，来自10(4)细胞的衍生物中导管发育不良的表达高于来自10(5)细胞的衍生物。当致癌物暴露和细胞解离之间经过 24 小时时，源自 10(4) 细胞的增生中病变的表达需要活跃的导管生长，因为发育中的增生中存在的病变被重塑，并且当增生完全填充脂肪垫时不再可检测到。当第二代移植物源自完全（非生长）第一代生长物的细胞时，导管发育不良被重新表达，但再次仅在发育中的生长物内表达。增加致癌物治疗和细胞解离之间的时间，即6天或更长，会导致导管发育不良的频率增加，并导致大量病变在完全生长过程中持续存在。这些结果表明，获得导致导管发育不良的生长潜力改变以及这些病变从生长调节机制中获得一定自主性的能力是在致癌剂治疗后不同时间发生的独立事件。
The expression of mammary ductal dysplasia has been shown to be enhanced in mammary outgrowths derived from enzymatically dissociated mammary cells and influenced by the number of cells used to derive the outgrowths. The present study was designed to examine this cell dose effect further and to determine if the developmental state of the outgrowths or the time between carcinogen administration and cell dissociation affects the expression and persistence of the ductal dysplasias. Mammary outgrowths were derived by injecting 10(4) or 10(5) enzymatically dissociated mammary cells into gland-free fat pads of 3-week-old female BALB/c mice. Donor animals were untreated or were exposed to either 7,12-dimethylbenz(a)anthracene or gamma-ray irradiation. The outgrowths were examined at 4.5, 8, 10, or 16 weeks after transplantation, depending on the experiment, and classified as having normal or dysplastic growth. The data indicated that expression of ductal dysplasia was greater in outgrowths derived from 10(4) than from 10(5) cells regardless of the developmental state of the outgrowths. When 24 hr elapsed between carcinogen exposure and cell dissociation, expression of lesions in outgrowths derived from 10(4) cells required active ductal growth, in that lesions that were present in developing outgrowths remodeled and were no longer detectable when the outgrowths completely filled the fat pad. When second-transplant-generation outgrowths were derived from cells of full (non-growing) first-generation outgrowths, ductal dysplasias were reexpressed but, once again, only within developing outgrowths. Increasing the time between carcinogen treatment and cell dissociation, i.e., 6 days or longer, resulted in both increased frequencies of ductal dysplasias and substantial numbers of lesions which persisted within full outgrowths. These results suggested that the acquisition of the altered growth potential which resulted in ductal dysplasias and the ability of these lesions to gain some autonomy from growth-regulatory mechanisms were separate events that occurred at different times after carcinogen treatment.
DOI：
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发表时间：
1981
期刊：
The American journal of pathology
影响因子：
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作者：
Becker,FF
通讯作者：
Becker,FF