Clonal analysis of epidermal patterning during maize leaf development
Clonal analysis of epidermal patterning during maize leaf development
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DOI：
10.1006/dbio.1999.9429
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发表时间：
1999-12-15
影响因子：
2.7
通讯作者：
Smith, LG
中科院分区：
文献类型：
--
作者：
Hernandez, ML;Passas, HJ;Smith, LG
在植物中，特化的表皮细胞以半有序的方式排列。在玉米等禾本科植物中，气孔和其他特化细胞类型在叶表皮内以线性模式分化。已经提出了多种机制来指导表皮细胞的分化模式。一类模型提出，细胞分化的模式取决于表皮细胞之间的谱系关系。另一类模型提出，表皮图案取决于位置信息，而不是血统关系。在双子叶植物的表皮中，细胞谱系是影响气孔形成的重要因素，而不是毛状体。在这项研究中，细胞谱系在玉米叶表皮气孔和泡状细胞线形模式中的作用进行了研究。幼叶的表皮细胞克隆通过从gl15-m中切除dSpm来标记，而在成年叶片中通过从bz2-m中切除ds2来标记。分析了这些克隆与气孔和泡状细胞模式的关系，测试了克隆起源假说对这些细胞类型模式的具体预测。我们发现，绝大多数被分析的克隆未能满足这些预测。我们的结果清楚地表明，谱系不能解释气孔和泡状细胞的线性模式，这意味着位置信息必须指导这些细胞类型在玉米中的分化模式。(C)1999年学术出版社。
In plants, specialized epidermal cells are arranged in semiordered patterns. In grasses such as maize, stomata and other specialized cell types differentiate in linear patterns within the leaf epidermis. A variety of mechanisms have been proposed to direct patterns of epidermal cell differentiation. One class of models proposes that patterns of cellular differentiation depend on the lineage relationships among epidermal cells. Another class of models proposes that epidermal patterning depends on positional information rather than lineage relationships. In the dicot epidermis, cell lineage is an important factor in the patterning of stomata, but not trichomes. In this study, the role of cell lineage in the linear patterning of stomata and bulliform cells in the maize leaf epidermis is investigated. Clones of epidermal cells in juvenile leaves were marked by excision of dSpm from gl15-m and in adult leaves by excision of Ds2 from bz2-m. These clones were analyzed in relation to patterns of stomata and bulliform cells, testing specific predictions of clonal origin hypotheses for the patterning of these cell types. We found that the great majority of clones analyzed failed to satisfy these predictions. Our results clearly show that lineage does not account for the linear patterning of stomata and bulliform cells, implying that positional information must direct the differentiation patterns of these cell types in maize. (C) 1999 Academic Press.