Multiple biomarkers of biological effects induced by cadmium in clam Ruditapes philippinarum
Multiple biomarkers of biological effects induced by cadmium in clam Ruditapes philippinarum
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DOI：
10.1016/j.fsi.2015.03.024
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发表时间：
2015-06-01
影响因子：
4.7
通讯作者：
Zhao, Jianmin
中科院分区：
文献类型：
--
作者：
Ji, Chenglong;Wu, Huifeng;Zhao, Jianmin
镉（Cd）是渤海已知的重金属污染物。菲律宾蛤仔是渤海沿岸的重要渔业品种。在本研究中，利用菲律宾蛤消化腺中的多种生化指标来表征两种浓度（20和200μg/L）Cd诱导的生物效应。血细胞总数、活性氧产生和抗氧化酶活性表明，镉在蛤消化腺中诱导剂量依赖性免疫和氧化应激。代谢反应表明，两种镉暴露都会引起以支链氨基酸（缬氨酸、亮氨酸和异亮氨酸）升高为标志的免疫应激，以及能量代谢紊乱。与渗透压相关的差异代谢生物标志物，包括高碱、甜菜碱、酪氨酸和苯丙氨酸，表明蛤消化腺中镉暴露诱导的差异反应机制。此外，两种镉处理均通过不同的代谢途径增强了蛤消化腺的厌氧代谢。 (c) 2015 Elsevier Ltd. 保留所有权利。
Cadmium (Cd) is a known heavy metal pollutant in the Bohai Sea. Manila clam Ruditapes philippinarum is an important fishery species along the Bohai coast. In this study, the biological effects induced by two concentrations (20 and 200 mu g/L) of Cd were characterized using multiple biochemical indices in the digestive glands of clam R. philippinarum. The total hemocyte counts, reactive oxygen species productions and antioxidant enzyme activities exhibited that Cd induced dose-dependent immune and oxidative stresses in clam digestive glands. Metabolic responses indicated that both Cd exposures caused immune stress marked by the elevated branched chain amino acids (valine, leucine and isoleucine), together with the disturbance in energy metabolism. The differential metabolic biomarkers related to osmotic stress, including homarine, betaine, tyrosine and phenylalanine, suggested the differential responsive mechanisms in clam digestive glands induced by Cd exposures. In addition, both Cd treatments enhanced the anaerobiosis metabolism in clam digestive glands via differential metabolic pathways. (c) 2015 Elsevier Ltd. All rights reserved.