Characterisation of the mob locus of Rhodobacter sphaeroides WS8: mobA is the only gene required for molybdopterin guanine dinucleotide synthesis
Characterisation of the mob locus of Rhodobacter sphaeroides WS8: mobA is the only gene required for molybdopterin guanine dinucleotide synthesis
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球形红杆菌 WS8 mob 基因座的表征：mobA 是钼蝶呤鸟嘌呤二核苷酸合成所需的唯一基因
DOI：
10.1007/s002030100291
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发表时间：
2001
影响因子：
2.8
通讯作者：
T. Palmer
中科院分区：
文献类型：
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作者：
G. Buchanan;J. Kuper;R. Mendel;G. Schwarz;T. Palmer
抽象的。几种细菌的MOB基因参与了钼蝶呤向鸟嘌呤二核苷酸的转化。球形红杆菌WS8的mob基因由三个基因mobABC组成。已经构建了每个MOB基因的染色体框内缺失。MOBA突变株具有DMSO还原酶和周质硝酸还原酶的失活活性(这两种酶都是钼多宾鸟嘌呤二核苷酸需要酶)，但对钼多宾酶黄嘌呤脱氢酶的活性没有影响。通过对MOBA菌株细胞提取液中碘氧化后钼辅因子形式的分析，证实了MOBA突变株不能合成钼杂多酚鸟嘌呤二核苷酸。Mobb和mobC的突变不会损害钼酶的活性，并积累了野生型的钼蝶呤和钼多蝶呤鸟嘌呤二核苷酸，表明它们在钼辅助因子的合成中没有受到影响。在MOBA突变株中，在周质中发现了失活的DMSO还原酶，这表明钼辅助因子的插入不一定是出口的先决条件。
Abstract. The mob genes of several bacteria have been implicated in the conversion of molybdopterin to molybdopterin guanine dinucleotide. The mob locus of Rhodobacter sphaeroides WS8 comprises three genes, mobABC. Chromosomal in-frame deletions in each of the mob genes have been constructed. The mobA mutant strain has inactive DMSO reductase and periplasmic nitrate reductase activities (both molybdopterin guanine dinucleotide-requiring enzymes), but the activity of xanthine dehydrogenase, a molybdopterin enzyme, is unaffected. The inability of a mobA mutant to synthesise molybdopterin guanine dinucleotide is confirmed by analysis of cell extracts of the mobA strain for molybdenum cofactor forms following iodine oxidation. Mutations in mobB and mobC are not impaired for molybdoenzyme activities and accumulate wild-type levels of molybdopterin and molybdopterin guanine dinucleotide, indicating they are not compromised in molybdenum cofactor synthesis. In the mobA mutant strain, the inactive DMSO reductase is found in the periplasm, suggesting that molybdenum cofactor insertion is not necessarily a pre-requisite for export.
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Johnson,JL;Indermaur,LW;Rajagopalan,KV
通讯作者：
Rajagopalan,KV