Multiple inductive signals are involved in the development of the ctenophore Mnemiopsis leidyi
Multiple inductive signals are involved in the development of the ctenophore Mnemiopsis leidyi
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DOI：
10.1006/dbio.2001.0401
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发表时间：
2001-10-01
影响因子：
2.7
通讯作者：
Martindale, MQ
中科院分区：
文献类型：
--
作者：
Henry, JQ;Martindale, MQ
栉水母有八排纵向排列的梳状板纤毛。先前的细胞内细胞谱系分析表明，这些梳状排列来自两个胚胎谱系，都是四个e（1）微节的子代（e（11）和e（12））和四个m（1）微节的单个子代（m（12）微节）。虽然分离的e（1）微粒会自发产生梳板，但细胞缺失实验表明，在去除e（1）后代后的胚胎发生过程中不会出现梳板。因此，m（1）谱系需要e（1）谱系的诱导相互作用来促成梳板的形成。在这里，我们表明，虽然m（12）细胞通常是唯一的m（1）衍生物，有助于梳板的形成，m（12）细胞能够产生梳板在缺乏m（12）细胞。m（11）个细胞通常不形成梳状行的原因可以归因于它们相对于关键信号中心的更远的位置（例如，E（1）后代）或可由其它附近细胞如姐妹细胞M（12）提供的抑制信号。此外，我们表明，由e（1）谱系提供的信号是不足以为m（1）衍生的梳板形成。还需要E或M谱系的内中胚层后代（3E或2M大单体）提供的信号。(C)北京：科学出版社.
Ctenophores possess eight longitudinally arrayed rows of comb plate cilia. Previous intracellular cell lineage analysis has shown that these comb rows are derived from two embryonic lineages, both daughters of the four e(1) micromeres (e(11), and e(12)) and a single daughter of the four m(1) micromeres (the m(12) micromeres). Although isolated e(1) micromeres will spontaneously generate comb plates, cell deletion experiments have shown that no comb plates appear during embryogenesis following the removal of e(1) descendents. Thus, the m(1) lineage requires the inductive interaction of the e(1) lineage to contribute to comb plate formation. Here we show that, although m(12) cells are normally the only m(1) derivatives to contribute to comb plate formation, m(12) cells are capable of generating comb plates in the absence m(12) cells. The reason that m(11) cells do not normally make comb rows may be attributable either to their more remote location relative to critical signaling centers (e.g., e(1) descendants) or to inhibitory signals that may be provided by other nearby cells such as sister cells m(12). In addition, we show that the signals provided by the e(1) lineage are not sufficient for m(1)-derived comb plate formation. Signals provided by endomesodermal progeny of either the E or the M lineages (the 3E or 2M macromeres) are also required. (C) 2001 Academic Press.