Fermentative degradation of 3-hydroxybenzoate in pure culture by a novel strictly anaerobic bacterium, Sporotomaculum hydroxybenoicum gen. nov., sp. nov.
Fermentative degradation of 3-hydroxybenzoate in pure culture by a novel strictly anaerobic bacterium, Sporotomaculum hydroxybenoicum gen. nov., sp. nov.
复制标题
DOI：
10.1099/00207713-48-1-215
复制
发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Schink, B
中科院分区：
文献类型：
--
作者：
Brauman, A;M端ller, JA;Schink, B
从白蚁后肠匀浆中分离出一种严格厌氧细菌 BT 菌株，在纯培养物中分离出来，并以 3-羟基苯甲酸盐作为唯一的碳源和能量来源生长。没有其他测试底物被降解，硫酸盐、亚硫酸盐、硫代硫酸盐、硝酸盐、三价铁、氧或富马酸盐没有被还原，并且没有观察到向伙伴生物体的电子转移。 3-羟基苯甲酸酯发酵成丁酸盐、乙酸盐和CO2。在培养物上清液中检测到苯甲酸作为中间体。分离物是一种轻微能动的、形成内生孢子的革兰氏阳性杆状体； 16S rDNA 序列分析显示与 Desulfotomaculum 属成员高度相似。 DNA的G+C含量为48mol％，BT菌株由于缺乏异化硫酸盐还原而与Desulfotomaculum属的成员显着不同，因此被描述为新属和新种Sporotomaculum 羟基苯甲酸属的模式菌株。十一月，SP。十一月
A strictly anaerobic bacterium, strain BT, from termite hindgut homogenates, was isolated in pure culture and grew on 3-hydroxybenzoate as sole source of carbon and energy. No other substrate tested was degraded, sulfate, sulfite, thiosulfate, nitrate, ferric iron, oxygen or fumarate were not reduced, and no electron transfer to partner organisms was observed. 3-Hydroxybenzoate was fermented to butyrate, acetate and CO2. Benzoate was detected in the culture supernatant as an intermediate. The isolate was a slightly motile, endospore-forming Gram-positive rod; 16S rDNA sequence analysis revealed a high similarity to members of the genus Desulfotomaculum. The G+C content of the DNA was 48 mol%, Strain BT differs from the members of the genus Desulfotomaculum significantly due to its lack of dissimilatory sulfate reduction, and is therefore described as the type strain of a new genus and species, Sporotomaculum hydroxybenzoicum gen. nov., sp. nov.