The transcriptional landscape of dorsal root ganglia after sciatic nerve transection.
The transcriptional landscape of dorsal root ganglia after sciatic nerve transection.
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坐骨神经横断后背根神经节的转录景观
DOI：
10.1038/srep16888
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发表时间：
2015-11-18
影响因子：
4.6
通讯作者：
Gu X
中科院分区：
文献类型：
--
作者：
Li S;Xue C;Yuan Y;Zhang R;Wang Y;Wang Y;Yu B;Liu J;Ding F;Yang Y;Gu X
在周围神经损伤后，转录反应被精心安排以调节受损神经中许多基因的表达，从而激活内在再生程序。为了更好地了解周围神经再生的分子调控，我们旨在研究大鼠坐骨神经切断后背根神经节（DRG）的转录景观。cDNA微阵列分析被用来确定成千上万的基因差异表达在不同的时间点后神经损伤（PNI）。Euclidean距离矩阵、主成分分析和系统聚类分析结果表明，在14 d PNI期间，基因表达的2个时间节点转换可以分离3个不同的转录时相。通过对神经损伤后变化的形态学观察，将这3个阶段分别命名为“应激反应阶段”、“预再生阶段”和“再生阶段”。基因本体分析揭示了每个转录阶段的生物学过程、细胞成分和分子功能的不同特征。此外，IntimidityPathway分析表明，差异表达的基因，主要是转录因子和与轴突/轴突生长相关的基因，可能会整合到调控网络中，以高度合作的方式介导周围神经再生的调控。
Following peripheral nerve injury, transcriptional responses are orchestrated to regulate the expression of numerous genes in the lesioned nerve, thus activating the intrinsic regeneration program. To better understand the molecular regulation of peripheral nerve regeneration, we aimed at investigating the transcriptional landscape of dorsal root ganglia (DRGs) after sciatic nerve transection in rats. The cDNA microarray analysis was used to identify thousands of genes that were differentially expressed at different time points post nerve injury (PNI). The results from Euclidean distance matrix, principal component analysis and hierarchical clustering indicated that 2 nodal transitions in temporal gene expressions could segregate 3 distinct transcriptional phases within the period of 14 d PNI. The 3 phases were designated as “a stress response phase”, “a pre-regeneration phase” and “a regeneration phase”, respectively, by referring to morphological observation of post-nerve-injury changes. The gene ontology (GO) analysis revealed the distinct features of biological process, cellular component and molecular function at each transcriptional phase. Moreover, Ingenuity Pathway Analysis suggested that differentially expressed genes, mainly transcription factors and genes associated with neurite/axon growth, might be integrated into regulatory networks to mediate the regulation of peripheral nerve regeneration in a highly cooperative manner.