MVE1, Encoding the Velvet Gene Product Homolog in Mycosphaerella graminicola, Is Associated with Aerial Mycelium Formation, Melanin Biosynthesis, Hyphal Swelling, and Light Signaling
MVE1, Encoding the Velvet Gene Product Homolog in Mycosphaerella graminicola, Is Associated with Aerial Mycelium Formation, Melanin Biosynthesis, Hyphal Swelling, and Light Signaling
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DOI：
10.1128/aem.01830-10
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发表时间：
2011-02-01
影响因子：
4.4
通讯作者：
Goodwin, Stephen B.
中科院分区：
文献类型：
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作者：
Choi, Yoon-E;Goodwin, Stephen B.
禾生球腔菌（Mycosphaerella graminicola）是引起小麦壳针孢斑病的重要病原菌。尽管M.尽管禾本科生在全球小麦生产中起重要作用，但对其分子生物学的了解有限。天鹅绒基因veA是多种细胞过程的关键调节因子之一，包括许多真菌的发育和次生代谢。然而，迄今为止分析的物种与Dothideomycetes（最大的一类植物病原真菌）无关，并且veA在该组中的功能尚不清楚。为了验证天鹅绒基因在Dothideomycetes中具有相似功能的假设，鉴定了veA同源基因MVE 1，并在M中产生基因缺失突变（Delta mve 1）。草生菌。所有的MVE 1突变体表现出一致的多效性表型，表明MVE 1参与多种信号通路。Delta mve 1菌株显示白化病表型，黑色素生物合成显著减少，琼脂平板上气生菌丝体产量减少。在液体培养中，Delta mve 1菌株表现出异常的菌丝肿胀，这是完全抑制渗透胁迫或较低的温度。此外，MVE 1基因缺失导致对摇动的超敏反应、疏水性降低以及对气生菌丝体生产的光依赖性刺激的盲目性。然而，在Delta mve1菌株中，致病性没有改变。因此，与MVE 1相关的光信号传导途径似乎对三孢壳针孢斑点病并不重要。我们的数据表明，MVE1基因在多个关键信号通路中起着至关重要的作用，并与M.草生菌。
The ascomycete fungus Mycosphaerella graminicola is an important pathogen of wheat that causes Septoria tritici blotch. Despite the serious impact of M. graminicola on wheat production worldwide, knowledge about its molecular biology is limited. The velvet gene, veA, is one of the key regulators of diverse cellular processes, including development and secondary metabolism in many fungi. However, the species analyzed to date are not related to the Dothideomycetes, the largest class of plant-pathogenic fungi, and the function of veA in this group is not known. To test the hypothesis that the velvet gene has similar functions in the Dothideomycetes, a veA-homologous gene, MVE1, was identified and gene deletion mutations (Delta mve1) were generated in M. graminicola. All of the MVE1 mutants exhibited consistent pleiotropic phenotypes, indicating the involvement of MVE1 in multiple signaling pathways. Delta mve1 strains displayed albino phenotypes with significant reductions in melanin biosynthesis and less production of aerial mycelia on agar plates. In liquid culture, Delta mve1 strains showed abnormal hyphal swelling, which was suppressed completely by osmotic stress or lower temperature. In addition, MVE1 gene deletion led to hypersensitivity to shaking, reduced hydrophobicity, and blindness to light-dependent stimulation of aerial mycelium production. However, pathogenicity was not altered in Delta mve1 strains. Therefore, the light-signaling pathway associated with MVE1 does not appear to be important for Septoria tritici blotch disease. Our data suggest that the MVE1 gene plays crucial roles in multiple key signaling pathways and is associated with light signaling in M. graminicola.