Intact Chemoreceptor‐Based Biosensors
Intact Chemoreceptor‐Based Biosensors
复制标题
基于完整化学感受器的生物传感器
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
G. Rechnitz
中科院分区：
文献类型：
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作者：
Todd Q. Barker;R. Buch;G. Rechnitz
提出了一种受体生物传感器，它使用来自夏威夷梭子蟹物种 Por‐tunis sanguinolentus 触角结构的完整的基于化学感受器的分子识别。该传感器耦合到学习、模式识别计算以执行分析化学。动作电位波形用于确定与特定分析物相关的各个动作电位类型的身份。使用的模式识别计算称为聚类分析（CA）和主成分分析（PCA）。通过使用聚类分析结果的树状图来确定动作电位相似性，并通过使用主成分得分图来进一步阐明。对分析物和背景响应进行分类后进行定量分析。研究人员对盐度和三甲胺 N-氧化物（甲壳动物生活环境中发现的两种化学成分）的化学反应进行了研究，并给出了几个数量级的分析反应。
A receptrode biosensor is presented that uses intact chemoreceptor‐based molecular recognition from antennular structures of the Hawaiian swimming crab species Por‐tunis sanguinolentus. The sensor is coupled to a learning, pattern recognition calculation for performing analytical chemistry. Action potential waveforms are used to establish the identity of individual action potential types that can be associated to particular analytes. The pattern recognition calculations used are referred to as cluster analysis (CA) and principal component analysis (PCA). Action potential similarities are determined by using a dendrogram plot of the cluster analysis results and further elucidated by using principal component scores plots. Quantitative analysis was performed after classification of analyte and background responses. Chemoresponses to salinity and trimethylamine N‐oxide, two chemical constituents that are found in the crustacean living environment, were investigated and gave analytic responses over several orders of magnitude.