Dual-Site Fluorescent Probe for Visualizing the Metabolism of Cys in Living Cells
Dual-Site Fluorescent Probe for Visualizing the Metabolism of Cys in Living Cells
复制标题
用于可视化活细胞中半胱氨酸代谢的双位点荧光探针
DOI：
10.1021/jacs.6b12845
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发表时间：
2017-03-01
影响因子：
15
通讯作者：
Yin, Caixia
中科院分区：
文献类型：
--
作者：
Yue, Yongkang;Huo, Fangjun;Yin, Caixia
荧光探针作为一种非侵入性的生物分子代谢可视化工具，在研究生物分子的生理和病理过程中具有巨大的潜力。然而，对于半胱氨酸（Cys），没有一个报道的荧光探针可以成像活细胞中的代谢过程。为了实现这一目标，我们开发了一种香豆素衍生物的基础上合理设计的双识别位点的半胱氨酸及其代谢产物，SO2。该探针显示了两个不同的通道，分别对Cys和SO2进行荧光发射，并成功地应用于A549细胞和斑马鱼的成像。此外，与可逆的荧光响应对半胱氨酸，探针可以成像的酶促转化半胱氨酸到SO2在活的A549细胞中的比例的方式。目前的工作报告的第一个探针，以图像的内源性产生的SO2没有孵育的SO2供体。
Fluorescent probes, as noninvasive tools for visualizing the metabolism of biomolecules, hold great potential to explore their physiological and pathological processes. For cysteine (Cys), however, none of the reported fluorescent probes could image the metabolic processes in living cells. To achieve this goal, we developed a coumarin derivative based on rational design of the dual recognition sites for Cys and its metabolite, SO2. The probe displayed distinct two channels with turn-on fluorescent emission toward Cys and SO2, which were successfully applied for imaging both A549 cells and zebrafish. Further, with reversible fluorescent responses toward Cys, the probe could image the enzymatic conversion of Cys to SO2 in living A549 cells in a ratiometric manner. The present work reports the first probe to image the endogenous generated SO2 without incubation of the SO2 donors.