Significance of Achromobacter nematophilus Poinar and Thomas (Achromobacteraceae: Eubacteriales) in the development of the nematode, DD-136 (Neoaplectana sp. Steinernematidae)
Significance of Achromobacter nematophilus Poinar and Thomas (Achromobacteraceae: Eubacteriales) in the development of the nematode, DD-136 (Neoaplectana sp. Steinernematidae)
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DOI：
10.1017/s0031182000070980
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发表时间：
1966-05
期刊：
影响因子：
2.4
通讯作者：
G. Poinar;G. Thomas
中科院分区：
文献类型：
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作者：
G. Poinar;G. Thomas
在实验室无菌条件下，分别培养大蜡螟Galleria mellonella、Neoaplectana sp.（DD-136）和嗜热无色杆菌Achromobacter philophilus，研究线虫寄生过程中细菌与线虫的关系。感染期的线虫幼虫在没有A.嗜酸乳杆菌或任何其他细菌。然而，如果没有伴随的细菌，线虫就无法繁殖。只有当A.在血液中添加嗜热链球菌或可能的替代物，如铜绿假单胞菌，进行繁殖。A.嗜热链球菌和线虫被认为是互利共生的，因为细菌在线虫的自由生活阶段的肠内生活并受到保护，并由线虫运输和释放到宿主昆虫的血淋巴中。反过来，线虫依赖细菌繁殖。
By culturing Galleria mellonella, Neoaplectana sp. (DD-136) and Achromobacter nematophilus separately under axenic conditions in the laboratory, it was possible to study the relationship between the bacterium and nematode during nematode parasitism of the insect. The infective-stage juveniles of the nematode were able to penetrate and kill the insect host without the presence of A. nematophilus or any other bacterium. However, without accompanying bacteria the nematode was unable to reproduce. Only when A. nematophilus or a possible replacement, such as Pseudomonas aeruginosa, was added to the blood did reproduction occur. The relationship between A. nematophilus and the nematode is considered a mutualistic one, since the bacterium lives and is protected inside the intestine of the free-living stage of the nematode and is transported and released by the nematode to the haemolymph of a host insect. The nematode, in turn, is dependent on the bacterium for reproduction.