Pharmacokinetic study of ginsenoside Re with pure ginsenoside Re and ginseng berry extracts in mouse using ultra performance liquid chromatography/mass spectrometric method
Pharmacokinetic study of ginsenoside Re with pure ginsenoside Re and ginseng berry extracts in mouse using ultra performance liquid chromatography/mass spectrometric method
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DOI：
10.1016/j.jpba.2009.08.013
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发表时间：
2010-01-05
影响因子：
3.4
通讯作者：
Lim, Kyung-Min
中科院分区：
文献类型：
--
作者：
Joo, Kyung-Mi;Lee, Ji-Hae;Lim, Kyung-Min
人参皂苷Re是人参果提取物中的主要成分，采用超高效液相色谱-质谱（UPLC/MS）联用技术，同时测定小鼠血清中Re、Rg 1和Rh 1的浓度，研究了小鼠静脉注射和口服10和50 mg/kg纯Re或人参果提取物后的药代动力学。通过蛋白沉淀预处理血清样品，并在AQUITY UPLC BEH C-18色谱柱上使用5 mM甲酸铵和乙腈的移动的流动相进行梯度洗脱进行色谱分离。分析物和地高辛（I.S.）采用电喷雾负离子质谱法在选择离子监测模式下对人参皂苷Re进行分析鉴定，线性浓度范围为5.0-5000 ng/mL，检测下限低于2.5 ng/mL（i. v.给药后，雄性小鼠为0.2 +/- 0.03 h，雌性小鼠为0.5 +/- 0.08 h）口服吸收一般较差（F% 0.19-0.28）。值得注意的是，GB提取物显示出与纯B2 Re相同剂量的B2 Re的上级口服吸收（F% 0.33-0.75），表明GB提取物可能是B2 Re摄入的良好形式。(C)2009 Elsevier B. V.保留所有权利。
Ginsenoside Re is the major ginsenoside in ginseng berry(CB) extract and its pharmacokinetics were studied following the intravenous and oral administration of pure Re or ginseng berry extract in mouse with doses of 10 and 50 mg/kg using ultra performance liquid chromatography mass spectrometric (UPLC/MS) method which can simultaneously determine ginsenoside Re, Rg1 and Rh1 in mouse serum. The serum samples were pretreated by protein precipitation and chromatographic separation was performed on AQUITY UPLC BEH C-18 column using gradient elution with the mobile phase of 5 mM ammonium formate and acetonitrile. Analytes and digoxin (I.S.) were analyzed and identified using an electrospray negative ionization mass spectrometry in the selected ion monitoring mode with the linear concentration range of 5.0-5000 ng/mL and lower limits of detection (LLOD) under 2.5 ng/mL Ginsenoside Re was rapidly cleared from the body with a short half-life (0.2 +/- 0.03 h for male and 0.5 +/- 0.08 h for female mice after i.v.) and oral absorption was generally poor (F% 0.19-0.28). Notably, GB extract showed a superior oral absorption of ginsenoside Re (F% 0.33-0.75) at equivalent ginsenoside Re dose to pure ginsenoside Re, indicating that GB extract might be a good form for ginsenoside Re intake. (C) 2009 Elsevier B.V. All rights reserved.