Preparative Isolation and Purification of the Lignan Pinoresinol Diglucoside and Liriodendrin from the Bark of Eucommia Ulmoides Oliv. by High Speed Countercurrent Chromatography
Preparative Isolation and Purification of the Lignan Pinoresinol Diglucoside and Liriodendrin from the Bark of Eucommia Ulmoides Oliv. by High Speed Countercurrent Chromatography
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DOI：
10.1080/10826070601036324
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发表时间：
2007-01
影响因子：
1.3
通讯作者：
Suo-Min Feng;Shi-feng Ni;Wen‐ji Sun
中科院分区：
文献类型：
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作者：
Suo-Min Feng;Shi-feng Ni;Wen‐ji Sun
本文报道了一种从中药材杜仲中高通量分离松脂醇二葡萄糖苷(PDG)和马钱子苷(SDG)的方法。采用高速逆流层析。以正丁醇-冰醋酸-水(4：1：5，v/v/v)为流动相，流动相为下相，流速为2.0mL.m in−-1，采用头尾洗脱方式。该方法从200 mg粗提物中一步得到纯度为96.7%的PDG 21 mg，纯度95.5%的马钱子苷16 mg。采用高效液相色谱(HPLC)分析，结合质谱仪(MS)、红外光谱(IR)、核磁共振(NMR)等分析手段，对分离得到的化合物进行了纯度和结构鉴定。
Abstract In this study, we reported a high throughout isolation procedure of pinoresinol diglucoside (PDG) and liriodendrin (SDG) from the traditional Chinese medical plant Eucommia ulmoides Oliv. by high speed countercurrent chromatography. The solvent system consisted of n‐butanol‐acetic acid‐water(4∶1∶5, v/v/v), in which the lower phase was used as the mobile phase at a flow‐rate of 2.0 mL · min−1 in the head to tail elution mode. The method yielded 21 mg of PDG at 96.7% purity and 16 mg of liriodendrin at 95.5% purity from 200 mg of crude extract in a one step. The purity and identity of the isolated compounds were checked by high performance liquid chromatography (HPLC) analysis, in combination with mass spectrometry (MS), infrared spectrum (IR), and NMR measurements.