VALIDATION OF FLUORESCENT-LABELED MICROSPHERES FOR MEASUREMENT OF REGIONAL ORGAN PERFUSION
VALIDATION OF FLUORESCENT-LABELED MICROSPHERES FOR MEASUREMENT OF REGIONAL ORGAN PERFUSION
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DOI：
10.1152/jappl.1993.74.5.2585
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发表时间：
1993-05-01
影响因子：
3.3
通讯作者：
BRINKLEY, M
中科院分区：
文献类型：
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作者：
GLENNY, RW;BERNARD, S;BRINKLEY, M
用荧光标记微球估计狗肺、猪心脏和猪肾的局部灌注与用标准放射性标记微球获得的测量结果进行比较。将一对放射性和荧光标记的微球（15微米直径，6种颜色）注射到一只仰卧麻醉犬的中心静脉和三只仰卧麻醉猪的左心室，同时从猪的股动脉中取出参考血液样本。肺被切成约2 cm3的小块（n = 1,510）。每只猪心脏和肾脏切成约1g块（共n = 192和120）。每个器官和参考血液样本的放射性使用闪烁计数器测定，计数率根据衰变、背景和溢出进行校正。组织样本和参考血样用KOH消化过滤后，用溶剂提取荧光染料，或者不过滤从肺组织中提取荧光染料。使用自动分光光度计测定每个样品的每种颜色的荧光。从放射性和荧光两方面计算每个器官的灌注。放射性标记微球与荧光标记微球测定的流量相关性如下：r = 0.98 +/- 0.01 (SD)（肺，过滤，n = 588）， r = 0.99 +/- 0.00（肺，未过滤，n = 710）， r = 0.95 +/- 0.02（心脏，过滤），r = 0.96 +/- 0.02（肾，过滤）。与有色微球相比，荧光标记微球的定量方法更灵敏，劳动强度更低，成本更低。荧光标记微球为局部器官灌注的单次和重复测量提供了一种新的无放射性方法。
Estimations of dog lung, pig heart, and pig kidney regional perfusion by use of fluorescent-labeled microspheres were compared with measurements obtained with standard radiolabeled microspheres. Pairs of radio- and fluorescent-labeled microspheres (15 mum diam, 6 colors) were injected into a central vein of a supine anesthetized dog and the left ventricle of three supine anesthetized pigs while reference blood samples were simultaneously withdrawn from a femoral artery in the pigs. The lungs were cubed into approximately 2 cm3 pieces (n = 1,510). Each pig heart and kidney was cubed into approximately 1-g pieces (total n = 192 and 120, respectively). The radioactivity of each organ piece and reference blood sample was determined using a scintillation counter with count rates corrected for decay, background, and spillover. Tissue samples and reference blood samples were digested with KOH and filtered and the fluorescent dye was extracted with a solvent, or the dye was extracted from lung tissue without filtering. The fluorescence of each sample was determined for each color by use of an automated spectrophotometer. Perfusion was calculated for each organ piece from both the radioactivity and fluorescence. Correlation between flow determined by radio- and fluorescent-labeled microspheres was as follows: r = 0.98 +/- 0.01 (SD) (lung, filtered, n = 588), r = 0.99 +/- 0.00 (lung, nonfiltered, n = 710), r = 0.95 +/- 0.02 (heart, filtered), and r = 0.96 +/- 0.02 (kidney, filtered). Compared with colored microspheres, methods for quantitating fluorescent-labeled microspheres are more sensitive, less labor intensive, and less expensive. Fluorescent-labeled microspheres provide a new nonradioactive method for single and repeated measurement of regional organ perfusion.