Somatic insertions and deletions shape the human antibody repertoire
Somatic insertions and deletions shape the human antibody repertoire
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DOI：
10.1006/jmbi.1999.3289
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发表时间：
1999-12-03
影响因子：
5.6
通讯作者：
Tomlinson, IN
中科院分区：
文献类型：
--
作者：
de Wildt, RMT;van Venrooij, WJ;Tomlinson, IN
我们对来自365个IgG（+）B细胞的重链和轻链基因进行了测序，发现24个（6. 5%）含有体细胞引入的插入或缺失。这些插入和缺失聚集在抗原结合位点中的“热点”处，并且经常导致产生典型环结构的新组合或完全新的环，其不存在于人种系库中，但与在其他物种中看到的那些相似。因此，除了体细胞点突变和受体编辑之外，体细胞插入和缺失提供了将结构多样性引入抗体的另一种机制，体细胞点突变和受体编辑分别对结构多样性具有小的（单个氨基酸变化）和大的（链置换）影响。(C)北京：科学出版社.
We have sequenced the heavy and light chain genes from 365 IgG(+) B cells and found that 24 (6.5 %) contain somatically introduced insertions or deletions. These insertions and deletions are clustered at "hot-spots" in the antigen-binding site and frequently result in the creation of new combinations of canonical loop structures or entirely new loops that are not present in the human germline repertoire, but are similar to those seen in other species. Somatic insertion and deletion therefore provides a further mechanism for introducing structural diversity into antibodies in addition to somatic point mutation and receptor editing, which have small (single amino acid changes) and large (chain replacement) impacts on structural diversity, respectively. (C) 1999 Academic Press.