Effect of estradiol and relaxin on growth of fibroblastic cells isolated from guinea pig mammary glands.
Effect of estradiol and relaxin on growth of fibroblastic cells isolated from guinea pig mammary glands.
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雌二醇和松弛素对豚鼠乳腺分离的成纤维细胞生长的影响。
DOI：
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发表时间：
1984
影响因子：
3.6
通讯作者：
R. R. Anderson
中科院分区：
文献类型：
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作者：
L. Sheffield;R. R. Anderson
从豚鼠乳腺中分离成纤维细胞，并在96孔培养板中生长。它们用猪松弛素（0.0、0.5、1.0或1.5微克/毫升）和雌二醇-17 β（0、200、400或600微克/毫升）的析因排列处理。加入氚标记的胸苷或尿苷至每孔25 nCi的最终活性，并将细胞在37 ℃下孵育48小时。然后将细胞收获到滤纸上并计数氚。对照（0.0微克/毫升松弛素和0微克/毫升雌二醇）掺入3.7 nCi的氚化胸苷和4.8 nCi的氚化尿苷。松弛素和雌二醇都改变了胸苷和尿苷的掺入。这两种激素之间也存在相互作用。不含雌二醇和1.5 μ g/ml松弛素的胸苷掺入量为对照组的129%。胸苷的最佳掺入发生在0.5微克/毫升松弛素和400微克/毫升雌二醇。这种激素组合的反应是对照组的145%。尿苷掺入遵循不同的模式。浓度为1.5微克/毫升的松弛素单独给药时的最佳反应为对照组的141%。松弛素和雌二醇用于尿苷掺入的最佳组合为1.5 μ g/ml松弛素和400 μ g/ml雌二醇，其响应为对照的156%。这些数据表明，松弛素和雌二醇改变乳腺成纤维细胞的DNA和RNA合成，因此可能是重要的控制乳腺基质的生长。
Fibroblasts were isolated from the mammary glands of guinea pigs and grown in 96-well culture plates. They were treated with a factorial arrangement of porcine relaxin (0.0, 0.5, 1.0 or 1.5 micrograms/ml) and estradiol-17 beta (0, 200, 400 or 600 pg/ml). Tritiated thymidine or uridine was added to a final activity of 25 nCi per well and the cells incubated at 37 degrees C for 48 h. Cells were then harvested onto filter paper and counted for tritium. Controls (0.0 micrograms/ml relaxin and 0 pg/ml estradiol) incorporated 3.7 nCi of tritiated thymidine and 4.8 nCi tritiated uridine. Both relaxin and estradiol altered the incorporation of thymidine and uridine. There was also an interaction between the two hormones. Thymidine incorporation with no estradiol and 1.5 micrograms/ml relaxin was 129% of controls. The optimum incorporation of thymidine occurred with 0.5 micrograms/ml relaxin and 400 pg/ml estradiol. This combination of hormones gave a response of 145% of controls. Uridine incorporation followed a different pattern. Relaxin alone at a concentration of 1.5 micrograms/ml gave a near-optimum response of 141% of control. The optimum combination of relaxin and estradiol for uridine incorporation was 1.5 micrograms/ml relaxin and 400 pg/ml estradiol, which gave a response of 156% of controls. These data indicated that relaxin and estradiol alter DNA and RNA synthesis in mammary fibroblasts and thus may be important in controlling the growth of the mammary gland stroma.