Contrast-Enhanced X-ray Detection of Microcalcifications in Radiographically Dense Mammary Tissue Using Targeted Gold Nanoparticles
Contrast-Enhanced X-ray Detection of Microcalcifications in Radiographically Dense Mammary Tissue Using Targeted Gold Nanoparticles
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DOI：
10.1021/acsnano.5b02749
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发表时间：
2015-09-01
期刊：
影响因子：
17.1
通讯作者：
Roeder, Ryan K.
中科院分区：
文献类型：
--
作者：
Cole, Lisa E.;Vargo-Gogola, Tracy;Roeder, Ryan K.
乳腺密度降低了乳房X光检查的准确性，促使方法提高检测致密乳腺组织内异常的灵敏度和特异性，但缺乏临床前动物模型。因此，本研究的目的是研究放射学致密乳腺组织的小鼠模型和通过靶向递送双膦酸盐官能化金纳米颗粒（BP-Au NPs）对致密乳腺组织中微钙化的对比增强X射线检测。小鼠乳腺肿瘤病毒-多瘤病毒中T抗原（MMTV-PyMT或PyMT）模型中的乳腺（MG）随着年龄的增长表现出更大的放射学密度，并且与6-10周龄时的菌株和年龄匹配的野生型（WT）对照相比。PyMT小鼠中MG的更大射线照相密度掩盖了在WT小鼠的MG中以其他方式可检测到的微钙化的射线照相检测。然而，BP-Au纳米颗粒提供了增强的对比度，用于检测乳房内递送后通过计算机断层扫描测量的放射学致密（PyMT）和WT乳腺组织中的微钙化。BP-Au纳米颗粒靶向微钙化，以增强与周围乳腺组织的X射线对比度，从而提高了放射学致密乳腺组织检测的灵敏度和特异性。
Breast density reduces the accuracy of mammography, motivating methods to improve sensitivity and specificity for detecting abnormalities within dense breast tissue, but preclinical animal models are lacking. Therefore, the objectives of this study were to investigate a murine model of radiographically dense mammary tissue and contrast-enhanced X-ray detection of microcalcifications in dense mammary tissue by targeted delivery of bisphosphonate-functionalized gold nanoparticles (BP-Au NPs). Mammary glands (MGs) in the mouse mammary tumor virus - polyomavirus middle T antigen (MMTV-PyMT or PyMT) model exhibited greater radiographic density with age and compared with strain- and age-matched wild-type (WT) controls at 6-10 weeks of age. The greater radiographic density of MGs in PyMT mice obscured radiographic detection of microcalcifications that were otherwise detectable in MGs of WT mice. However, BP-Au NPs provided enhanced contrast for the detection of microcalcifications in both radiographically dense (PyMT) and WT mammary tissues as measured by computed tomography after intramammary delivery. BP-Au NPs targeted microcalcifications to enhance X-ray contrast with surrounding mammary tissue, which resulted in improved sensitivity and specificity for detection in radiographically dense mammary tissues.