EXPOSURE TO ALTERNATING HYPOXIA AND HYPEROXIA CAUSES SEVERE PROLIFERATIVE RETINOPATHY IN THE NEWBORN RAT
EXPOSURE TO ALTERNATING HYPOXIA AND HYPEROXIA CAUSES SEVERE PROLIFERATIVE RETINOPATHY IN THE NEWBORN RAT
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DOI：
10.1203/00006450-199412000-00007
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发表时间：
1994-12-01
影响因子：
3.6
通讯作者：
TOLMAN, BL
中科院分区：
文献类型：
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作者：
PENN, JS;HENRY, MM;TOLMAN, BL
最近的研究表明，暴露于可变的高氧比暴露于持续的高氧在新生大鼠中产生增生性视网膜病变方面更有效。为了在我们的研究中纳入更具临床相关性的氧气暴露范例，我们现在使用了50%至10%氧气的循环，并将其效果与使用新的暴露于先前使用的80/40%循环的效果进行了比较。从出生开始并持续14天，大鼠暴露于每24小时循环50 - 10%氧气或80 - 40%氧气的环境中。暴露后，处死一些大鼠以评估视网膜血管发育。其他人被转移到室内空气中4天，然后杀死和评估异常新生血管的存在-一个临床后果被认为是促进终止氧疗。与80/40%暴露组相比，50/10%循环导致氧气期间视网膜血管发育的延迟更大。在室内空气中暴露后4 d，50/10%组大鼠视网膜前新生血管的发生率为97%，80/40%组为72%。显然，受试者接受的氧气总量在产生增殖性视网膜病变方面不如其施用的其他参数重要。此外，变化范围（两种情况下均为40%）不是控制特征。我们的研究结果表明，氧气水平的一致性和避免缺氧水平应是重要的关注新生儿氧疗。
Exposure to variable hyperoxia has recently been shown to be much more effective at producing proliferative retinopathy in the newborn rat than exposure to constant hyperoxia. To incorporate a more clinically relevant oxygen-exposure paradigm in our studies, we have now used a cycle between 50 and 10% oxygen and have compared its effects with those found using new exposures to the previously used 80/40% cycle. Starting at birth and continuing for 14 d, rats were exposed to environments that cycled between 50 and 10% oxygen or 80 and 40% oxygen every 24 h. After exposure, some rats were killed for assessment of retinal vascular development. Others were removed to room air for 4 d before killing and evaluation for the presence of abnormal neovascularization-a clinical consequence believed to be promoted by termination of oxygen therapy. The 50/10% cycle resulted in greater retardation of retinal blood vessel development during oxygen than that found in the 80/40% exposure group. After 4 d postexposure in room air, the incidence of preretinal neovascularization was 97% in the 50/10% rats and 72% in the 80/40% group. Clearly, the overall amount of oxygen the subject receives is less critical than other parameters of its administration in producing proliferative retinopathy. Also, the range of variation (40% in both cases) is not the controlling characteristic. Our results suggest that consistency of oxygen level and avoidance of hypoxic levels should be important concerns in neonatal oxygen therapy.