Proton NMR study of the [d(ACGTATACGT)]2-2echinomycin complex: conformational changes between echinomycin binding sites.
Proton NMR study of the [d(ACGTATACGT)]2-2echinomycin complex: conformational changes between echinomycin binding sites.
复制标题
[d(ACGTATACGT)]2-2棘霉素复合物的质子核磁共振研究：棘霉素结合位点之间的构象变化。
DOI：
10.1093/nar/20.10.2411
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发表时间：
1992
影响因子：
14.9
通讯作者：
Feigon,J
中科院分区：
文献类型：
--
作者：
Gilbert,DE;Feigon,J
用质子核磁共振研究了棘球菌素与DNA解聚体[d(ACGTATACGT)]2的相互作用。在CpG步骤中，棘霉素作为双嵌入剂协同结合。末端的A·T碱基对是Hoogsteen碱基对，但中心的四个A·T碱基对都不是Hoogsteen碱基对。然而，药物的结合诱导DNA解旋，DNA被传播到中心APT步骤。相对于游离DNA中的A·T碱基对，所有四个中心A·T碱基对都是不稳定的。此外，根据这些和我们实验室的其他结果，我们得出结论，稳定的Hoogsteen碱基对的形成可能不是体内相关的结构变化。在相邻ACGT结合位点之间传播的结构变化是双链的解离和结合位点之间碱基配对的不稳定。
The interactions of echinomycin and the DNA decamer [d(ACGTATACGT)]2were studied by proton NMR. Echinomycin binds cooperatively as a bisintercalator at the CpG steps. The terminal A·T base pairs are Hoogsteen base paired, but none of the four central A·T base pairs are Hoogsteen base paired. However, binding of the drug induces unwinding of the DNA which is propagated to the central ApT step. All four central A·T base pairs are destabilized relative to those in the free DNA. Furthermore, based on these and other results from our laboratory, we conclude that the formation of stable Hoogsteen base pairs may not be the relevant structural changein vivo. The structural changes propagated between adjacent ACGT binding sites are the unwinding of the duplex and destabilization of the base pairing between binding sites.