Growth rate regulation of 4.5 S RNA and M1 RNA the catalytic subunit of Escherichia coli RNase P
Growth rate regulation of 4.5 S RNA and M1 RNA the catalytic subunit of Escherichia coli RNase P
复制标题
DOI：
10.1006/jmbi.1996.0461
复制
发表时间：
1996-08-23
影响因子：
5.6
通讯作者：
Nilsson, L
中科院分区：
文献类型：
--
作者：
Dong, HJ;Kirsebom, LA;Nilsson, L
我们研究了大肠杆菌核糖核酸酶P的催化亚基4.5 S RNA和Fv Il RNA在不同生长条件下的表达。这两种RNA物种的丰度都随着生长速度的增加而增加。以每小时0.4倍的速度，每个细胞大约有450个4.5M1RNA分子和80个M1RNA分子，以每小时2.7%的倍增速度，每个细胞分别增加到5300个和1060个分子。负责ppGpp合成的两个基因relA和Spot的缺失不会影响任何一种RNA的合成速度。但是，FIS基因的缺失使得4.5M1RNA的表达不受生长速度的影响，但对M1RNA的表达影响不大。这些数据表明，S 4.5RNA和M1RNA基因的表达都受生长速度的调控，但不是通过相同的机制。4.5M1RNA的生长速率依赖性积累依赖于FIS介导的反式激活，而M1RNA的积累不受ppGpp或FIS的控制。(C)1996学术出版社有限公司。
We have studied the expression of 4.5 S RNA and Fv Il RNA, the catalytic subunit of Escherchia coli RNase P, under various growth conditions. Both RNA species increase in abundance as a function of growth rate. There are roughly 450 molecules of 4.5 S RNA and 80 molecules of M1 RNA per cell at 0.4 doubling per hour, and this is increased to 5300 and 1060 molecules per cell, respectively, at 2.7 doublings per hour. Deletion of both relA and spoT, the two genes that are responsible for synthesis of ppGpp, does not affect the rate of synthesis of either RNA species. However, deletion of fis renders the expression of 4.5 S RNA independent of growth rate, but has little effect on the expression of M1 RNA. These data suggest that the expression of both 4.5 S RNA and M1 RNA genes are growth-rate regulated, but not through the same mechanism. The growth-rate dependent accumulation of 4.5 S RNA depends on FIS-mediated trans-activation, whereas that of M1 RNA is not governed by ppGpp or by FIS. (C) 1996 Academic Press Limited.