Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters
Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters
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DOI：
10.15252/embj.201695020
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发表时间：
2016-11-15
期刊：
影响因子：
11.4
通讯作者：
Zentner, Gabriel E.
中科院分区：
文献类型：
--
作者：
Gruenberg, Sebastian;Henikoff, Steven;Zentner, Gabriel E.
Mediator是一种保守的、重要的转录辅助激活因子复合体，但由于全基因组芯片获得的全基因组结合模式数据不一致，其体内功能尚不清楚。在这里，我们使用CHEC-SEQ，一种与CHIP正交的方法，生成了介体与酵母基因组结合的高分辨率图谱。我们发现，在所有测试的条件下，Mediator都与上游激活序列(UAS)结合，而不是核心启动子或基因体。令人惊讶的是，只有一小部分基因的介体占有率与转录水平相关。使用相同的方法定位TFIID，我们发现TFIID与TFIID和SAGA依赖的基因都相关，并且TFIID和介体占有率是协同的。我们的结果阐明了介体招募和与基因组的结合，表明介体与UASs的结合是广泛的，部分与转录解偶联，部分由TFIID介导。
Mediator is a conserved, essential transcriptional coactivator complex, but its invivo functions have remained unclear due to conflicting data regarding its genome-wide binding pattern obtained by genome-wide ChIP. Here, we used ChEC-seq, a method orthogonal to ChIP, to generate a high-resolution map of Mediator binding to the yeast genome. We find that Mediator associates with upstream activating sequences (UASs) rather than the core promoter or gene body under all conditions tested. Mediator occupancy is surprisingly correlated with transcription levels at only a small fraction of genes. Using the same approach to map TFIID, we find that TFIID is associated with both TFIID- and SAGA-dependent genes and that TFIID and Mediator occupancy is cooperative. Our results clarify Mediator recruitment and binding to the genome, showing that Mediator binding to UASs is widespread, partially uncoupled from transcription, and mediated in part by TFIID.