Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells.
Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells.
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DOI：
10.1371/journal.pone.0193483
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Yuan Z
中科院分区：
文献类型：
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作者：
Guo W;Zhong K;Wei H;Nie C;Yuan Z
越来越多的证据表明，长链非编码RNA（lncRNA）在包括胰腺癌在内的人类癌症的发展中起着关键作用。长链非编码RNA SPRY 4-IT 1（sprouty 4-intron transcript 1）在多种人类肿瘤中具有致癌作用。然而，SPRY 4-IT 1在胰腺癌中的作用尚不清楚。本研究的目的是确定SPRY 4-IT 1在胰腺癌增殖和侵袭中的作用。本研究采用Real-time RT-PCR、Western blotting、MTT法、创伤愈合实验、Transwell实验和转染等方法，对SPRY 4-IT 1的功能和作用机制进行了深入研究。我们发现SPRY 4-IT 1的下调抑制胰腺癌细胞的生长并诱导细胞凋亡。此外，SPRY 4-IT 1基因敲低可诱导细胞周期停滞在G 0/G1期。此外，SPRY 4-IT 1的抑制延缓了胰腺癌细胞的细胞迁移和侵袭。SPRY 4-IT 1过表达可促进胰腺癌细胞的生长和侵袭，抑制细胞凋亡。在机制上，SPRY 4-IT 1的抑制抑制胰腺癌细胞中Cdc 20的表达。我们的研究结果表明，抑制SPRY 4-IT 1可能是治疗胰腺癌的一种潜在的治疗方法。
Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play a critical role in the development of human cancers including pancreatic cancer. Long non-coding RNA SPRY4-IT1 (sprouty4-intron transcript 1) has been reported to play an oncogenic role in various types of human carcinomas. However, the role of SPRY4-IT1 in pancreatic cancer is unclear. The objective of this study was to determine the function of SPRY4-IT1 on proliferation and invasion in pancreatic cancer. In the current study, we dissected the function and mechanism of SPRY4-IT1 by multiple approaches including Real-time RT-PCR, Western blotting analysis, MTT assay, Wound healing assay, Transwell assay, and transfection. We found that down-regulation of SPRY4-IT1 inhibited cell growth and induced cell apoptosis in pancreatic cancer cells. Moreover, SPRY4-IT1 knockdown induced cell cycle arrest at G0/G1 phase. Furthermore, inhibition of SPRY4-IT1 retarded cell migration and invasion in pancreatic cancer cells. Overexpression of SPRY4-IT1 enhanced cell growth and invasion, and inhibited cell apoptosis in pancreatic cancer cells. Mechanistically, suppression of SPRY4-IT1 inhibited the expression of Cdc20 in pancreatic cancer cells. Our findings demonstrated that inhibition of SPRY4-IT1 could be a potential therapeutic approach for the treatment of pancreatic cancer.