BIOSYNTHESIS OF NICOTINE ALKALOIDS IN NICOTIANA GLUTINOSA - INTERRELATIONSHIPS AMONG NICOTINE NORNICOTINE ANABASINE AND ANATABINE
BIOSYNTHESIS OF NICOTINE ALKALOIDS IN NICOTIANA GLUTINOSA - INTERRELATIONSHIPS AMONG NICOTINE NORNICOTINE ANABASINE AND ANATABINE
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DOI：
10.1016/0003-9861(65)90008-1
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发表时间：
1965-01-01
影响因子：
3.9
通讯作者：
RAPOPORT, H
中科院分区：
文献类型：
--
作者：
ALWORTH, WL;RAPOPORT, H
从C14 O2与完整的烟草植物和幼苗进行的生物合成已经证明，去甲烟碱不是尼古丁的前体在植物的根或地上部分。这一结论与用H_3对普通烟草生物碱进行提纯的结果相反。最可能的关系，基于C14 O2的生物合成和支持一些以前的结论，即降烟碱产生的尼古丁在根和地上部分的脱甲基。C14 O2生物合成后新木贼碱和安他宾的相对比活性表明新木贼碱不是新烟草碱的前体。在尼古丁，降烟碱，新烟草碱，和假木贼碱的情况下，更高的比活性，注意到在从根中分离的样品比从地上部分中分离的那些。这表明这4种生物碱都能在烟草根中形成，而且由于本实验中C14首先进入植株地上部分的代谢，这些结果表明N.甘草根是这些生物碱中每一种形成的最活跃部位。最后，对新烟碱、新烟草碱和尼古丁的比活性进行比较后发现，如果这些生物碱之间存在代谢产物-产物关系，那么它一定是尼古丁[前向箭头]新烟草碱或新烟碱，或两者兼而有之。
Biosyntheses from C14O2 carried out with both intact Nicotiana glutinosa plants and seedlings have demonstrated that nornicotine is not the precursor of nicotine in either the root or the aerial portions of the plant. This conclusion contrasts with that reached on the basis of H3 incorporations into the alkaloids of Nicotiana rustic a. The most probable relationship, based on C14O2 biosyntheses and supporting some previous conclusions, ie that nornicotine arises by demethylation of nicotine in both the root and aerial portions. The relative specific activities of anabasine and antabine after the C14O2 biosynthesis indicated that anabasine is not the precursor of anatabine. In the case of nicotine, nornicotine, anatabine, and anabasine, higher specific activities were noted in the samples isolated from the root than in those isolated from the aerial portions. This shows that these 4 alkaloids all can be formed in the Nicotiana root, and furthermore, since C14 in these experiments first enters into the metabolism of the aerial portion of the plants, these results indicate that the N. glutinosa root is the most active site of formation of each of these alkaloids. Finally, a comparison of the specific activities of anabasine, anatabine, and nicotine suggests that if a precursor-product relationship exists among these alkaloids it must be nicotine [forward arrow] anatabine or anabasine, or both.