Delta-9-tetrahydrocannabinol increases prodynorphin and proenkephalin gene expression in the spinal cord of the rat
Delta-9-tetrahydrocannabinol increases prodynorphin and proenkephalin gene expression in the spinal cord of the rat
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DOI：
10.1016/s0024-3205(97)00405-0
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发表时间：
1997-06-20
期刊：
影响因子：
6.1
通讯作者：
Manzanares, J
中科院分区：
文献类型：
--
作者：
Corchero, J;Avila, MA;Manzanares, J
已发现大麻类药物引起的痛觉减退与阿片系统有关。观察Delta-9-四氢大麻酚(THC)处理5天对雄性大鼠脊髓前强啡肽(PDYN)和前脑啡肽原(PENK)基因表达的影响。用Northern印迹分析法检测PDYN和PENK基因在整个脊髓(包括核周)的表达水平。THC亚慢性治疗(5 mg/kg/d；5天；i.p.)与赋形剂处理组相比，PDYN(39%)和Penk(34%)基因表达增加。这些结果表明，脊髓中THC的作用涉及阿片类药物活性的增加，因此支持该区域大麻素和阿片系统之间存在相互作用的假说。(C)1997年爱思唯尔科学公司。
Hypoalgesia induced by cannabinoid drugs has been found to implicate the opioid system. The effect of five days treatment with Delta-9-tetrahydrocannabinol (THC) was examined on prodynorphin (PDYN) and proenkephalin (PENK) gene expression in the spinal cord of male rats. PDYN and PENK gene expression was estimated measuring by northern blot analysis mRNA levels in the whole spinal cord, containing perikarya of these neurons. The subchronic treatment with THC (5 mg/kg/day; 5 days; i.p.) produced an increase in PDYN (39%) and PENK (34%) gene expression when compared with the vehicle treated group. These results suggest that the effects of THC in the spinal cord involve an increase in opioid activity, and therefore sustain the hypothesis of an interaction between the cannabinoid and opioid systems in this region. (C) 1997 Elsevier Science Inc.