Microfluidic single-particle chemical analyzer with dual-comb coherent Raman spectroscopy.
Microfluidic single-particle chemical analyzer with dual-comb coherent Raman spectroscopy.
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具有双梳相干拉曼光谱的微流控单颗粒化学分析仪。
DOI：
10.1364/ol.43.004057
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发表时间：
2018
期刊：
影响因子：
3.6
通讯作者：
K. Goda
中科院分区：
文献类型：
--
作者：
T. Ideguchi;Tasuku Nakamura;S. Takizawa;Miu Tamamitsu;Sangwook Lee;K. Hiramatsu;V. Ramaiah‐Badarla;Jee;Y. Kasai;T. Hayakawa;S. Sakuma;F. Arai;K. Goda
无标记颗粒分析是化学、制药和化妆品行业以及基础科学中的强大工具，但其通量明显低于基于荧光的同行。在这里，我们提出了一个无标记的单粒子分析仪的基础上，宽带双梳相干拉曼散射光谱工作在10 kHz的光谱扫描速率。作为一个概念验证的演示，我们进行宽带相干反斯托克斯拉曼散射测量的聚苯乙烯微粒上流动的声流控芯片在一个高吞吐量的>1000粒子每秒。这种高通量无标记颗粒分析仪具有对包括生物细胞在内的大量微粒进行高精度统计分析的潜力。
Label-free particle analysis is a powerful tool in chemical, pharmaceutical, and cosmetic industries as well as in basic sciences, but its throughput is significantly lower than that of fluorescence-based counterparts. Here we present a label-free single-particle analyzer based on broadband dual-comb coherent Raman scattering spectroscopy operating at a spectroscopic scan rate of 10 kHz. As a proof-of-concept demonstration, we perform broadband coherent anti-Stokes Raman scattering measurements of polystyrene microparticles flowing on an acoustofluidic chip at a high throughput of >1000 particles per second. This high-throughput label-free particle analyzer has the potential for high-precision statistical analysis of a large number of microparticles including biological cells.
影响因子：
64.8
作者：
Ideguchi, Takuro;Holzner, Simon;Haensch, Theodor W.
通讯作者：
Haensch, Theodor W.