In Vitro Studies on Regulation of Osteogenic Activities by Electrical Stimulus on Biodegradable Electroactive Polyelectrolyte Multilayers
In Vitro Studies on Regulation of Osteogenic Activities by Electrical Stimulus on Biodegradable Electroactive Polyelectrolyte Multilayers
复制标题
可生物降解电活性聚电解质多层电刺激调节成骨活性的体外研究
DOI：
10.1021/bm5007695
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发表时间：
2014-08-01
影响因子：
6.2
通讯作者：
Chen, Xuesi
中科院分区：
文献类型：
--
作者：
Cui, Haitao;Wang, Yu;Chen, Xuesi
本研究设计并采用层层组装法制备了一种新型的电活性四苯胺可降解聚合物多层膜涂层[（聚L-谷氨酸-接枝四苯胺/聚L-赖氨酸-接枝四苯胺）（n），（PGA-g-TA/PLL-g-TA）（n）]。与未接枝的PEM相比，四苯胺接枝的PEM在微/纳米结构中表现出更高的粗糙度和刚度。特殊的表面特性和典型的生物相容性更有利于前成骨细胞MC 3 T3-E1的粘附、增殖和分化。此外，观察到增强的调制的MC 3 T3-E1细胞分化为成熟的成骨细胞，当电活性PEMs与电刺激（ES）耦合时，特别是在成骨细胞分化的早期阶段。检测碱性磷酸酶（ALP）活性、钙沉积、免疫荧光染色和RT-qPCR对前体成骨细胞分化的影响。这些数据表明，电活性支架与电刺激耦合的综合效应更适合于开发生物电策略来控制细胞功能以促进骨再生。
In this study, a novel electroactive tetreaniline-containing degradable polyelectrolyte multilayer film (PEM) coating [(poly(L-glutamic acid)-graft-tetreaniline/poly(L-lysine)-graft-tetreaniline)(n), (PGA-g-TA/PLL-g-TA)(n)] was designed and fabricated by layer-by-layer (LbL) assembly method. Compared with the nongrafted PEMs, the tetreaniline-grafted PEMs showed higher roughness and stiffness in micro/nanoscale structures. The special surface characteristics and the typical electroconductive properties were more beneficial for adhesion, proliferation, and differentiation of preosteoblast MC3T3-E1 cells. Moreover, the enhanced effects were observed on the modulation of MC3T3-E1 cells that differentiated into maturing osteoblasts, when the electroactive PEMs were coupled with electrical stimulus (ES), especially in the early phase of the osteoblast differentiation. The alkaline phosphatase (ALP) activity, calcium deposition, immunofluorescence staining, and RT-qPCR were evaluated on the differentiation of preosteoblast. These data indicate that the comprehensive effects through coupling electroactive scaffolds with electrical stimulus are better to develop bioelectric strategies to control cell functions for bone regeneration.