Monoclonal antibodies to RT7 and LCA antigens in the rat: cell distribution and segregation analysis.
Monoclonal antibodies to RT7 and LCA antigens in the rat: cell distribution and segregation analysis.
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大鼠体内 RT7 和 LCA 抗原的单克隆抗体：细胞分布和分离分析。
DOI：
10.1089/hyb.1987.6.531
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Lubaroff,DM
中科院分区：
文献类型：
--
作者：
Mojcik,CF;Greiner,DL;Goldschneider,I;Lubaroff,DM
用单克隆抗体（mAb）研究了RT 7和白细胞共同抗原（L-CA）在大鼠体内的细胞分布和遗传关系。我们在这里证明，RT7.1同种异体抗原，识别的单克隆抗体BC 84，是存在于B淋巴细胞以及T淋巴细胞，虽然在明显不同的表观浓度; T细胞结合大约4倍以上的BC 84比B细胞。相比之下，NDS 58 mAb识别的多态性L-CA和OX 1 mAb识别的非多态性L-CA似乎在T和B细胞上以相等的浓度表达，并且其组织分布与RT 7.1同种异体抗原不同。我们还测试了一种名为8G6.1的新mAb，其与RT 7 b大鼠品系反应，并具有类似于多态性L-CA的细胞、组织和品系分布特征。分离分析的RT 7和多态性L-CA抗原系统使用一个单一的backeross模型表明，同种抗原识别的BC 84和NDS 58共分离，这两个是等位基因相对于8G6.1。由OX 1定义的L-CA抗原决定簇是非多态性的，因此与任何测试的抗原系统都不是等位基因。这些结果表明，RT 7和L-CA在大鼠中的表达之间存在密切的遗传关系。
Fluorescein-conjugated monoclonal antibodies (mAb) have been used to examine the cellular distribution and genetic relationship of the RT7 and the leukocyte common antigen (L-CA) in the rat. We demonstrate here that the RT7.1 alloantigen, recognized by the mAb BC84, is present on B lymphocytes as well as T lymphocytes, although at markedly different apparent concentrations; T cells binding approximately 4 times more BC84 than do B cells. In contrast, the polymorphic L-CA recognized by the NDS58 mAb and the non-polymorphic L-CA recognized by the OX1 mAb appear to be expressed in equal concentrations on T and B cells and differ in their tissue distribution from the RT7.1 alloantigen. We have also tested a new mAb termed 8G6.1, that is reactive with RT7b rat strains and has a cell, tissue and strain distribution profile resembling that of a polymorphic L-CA. Segregation analysis of the RT7 and polymorphic L-CA antigenic systems using a single backeross model demonstrate that the alloantigens recognized by BC84 and NDS58 cosegregate and that both are allelic with respect to 8G6.1. The L-CA antigenic determinant defined by OX1 was non-polymorphic and thus was not allelic with any of the antigenic systems tested. These results suggest that there is a close genetic relationship between the expression of RT7 and L-CA in the rat.