Surface acoustic wave actuated cell sorting (SAWACS)
Surface acoustic wave actuated cell sorting (SAWACS)
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DOI：
10.1039/b915522h
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Weitz, D. A.
中科院分区：
文献类型：
--
作者：
Franke, T.;Braunmueller, S.;Weitz, D. A.
我们描述了一种新型的微流控细胞分选仪，其以高分选率连续流动操作。该装置是基于表面声波细胞分选方案，并结合了荧光激活细胞分选（FACS）和荧光激活液滴分选（FADS）在微流体通道中的许多优点。它完全集成在PDMS设备上，并允许快速电子控制细胞转移。我们通过由表面声波激发的声流来引导细胞，该表面声波使流体偏转，而不依赖于偏转物体和连续相的材料性质的对比;因此，该装置的基本原理在没有通过用响应性标记物（例如磁性或可极化珠）预先标记细胞来额外增强分选的情况下起作用。单细胞以快至几kHz的速率直接从大量培养基中分选，而无需如传统FACS中那样预先包封到液滴隔室中。我们已经成功地指导HaCaT细胞（人角质形成细胞），小鼠成纤维细胞和MV 3黑色素瘤细胞。这种分选方法的低剪切力确保细胞在分选后存活。
We describe a novel microfluidic cell sorter which operates in continuous flow at high sorting rates. The device is based on a surface acoustic wave cell-sorting scheme and combines many advantages of fluorescence activated cell sorting (FACS) and fluorescence activated droplet sorting ( FADS) in microfluidic channels. It is fully integrated on a PDMS device, and allows fast electronic control of cell diversion. We direct cells by acoustic streaming excited by a surface acoustic wave which deflects the fluid independently of the contrast in material properties of deflected objects and the continuous phase; thus the device underlying principle works without additional enhancement of the sorting by prior labelling of the cells with responsive markers such as magnetic or polarizable beads. Single cells are sorted directly from bulk media at rates as fast as several kHz without prior encapsulation into liquid droplet compartments as in traditional FACS. We have successfully directed HaCaT cells (human keratinocytes), fibroblasts from mice and MV3 melanoma cells. The low shear forces of this sorting method ensure that cells survive after sorting.