Effect of retinoic acid on chondrocyte glycosaminoglycan biosynthsis
Effect of retinoic acid on chondrocyte glycosaminoglycan biosynthsis
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视黄酸对软骨细胞糖胺聚糖生物合成的影响
DOI：
10.1016/0003-9861(76)90325-8
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发表时间：
1976
影响因子：
3.9
通讯作者：
J. Poon
中科院分区：
文献类型：
--
作者：
S. Shapiro;J. Poon
研究了维甲酸对大鼠肋软骨软骨细胞体外合成糖胺聚糖的影响。在10−9至10−8m维甲酸水平下，糖胺聚糖对35so4的摄取减少了50%。在低维甲酸水平下，没有证据表明溶酶体酶释放。结果最好的解释是糖胺聚糖合成的修饰，而不是加速降解。在所研究的条件下，维甲酸选择性地修饰了35so4或[14C]葡萄糖胺掺入单个糖胺聚糖组分。放射性标记前体与硫酸肝素（和/或）肝素的相对掺入增加了三到四倍。硫酸软骨素6的放射性标记前体的相对掺入保持不变，而硫酸软骨素4和软骨素（和/或）透明质酸的掺入减少。在研究条件下，维甲酸似乎没有细胞毒性，并对糖胺聚糖的生物合成表现出选择性控制。这表明，在视黄醇水平高的维生素A症中，35so4进入糖胺聚糖的减少可能是由于维甲酸（一种自然产生的代谢物）水平低所致。
The effect of retinoic acid on glycosaminoglycan biosynthesis was investigated in rat costal cartilage chondrocytesin vitro. At levels of 10−9to 10−8mretinoic acid,35SO4uptake into glycosaminoglycans was reduced 50%. At these low levels of retinoic acid there was no evidence of lysosomal enzyme release. The results are explained best in terms of modification of glycosaminoglycan synthesis, rather than accelerated degradation. Retinoic acid selectively modified the incorporation of35SO4or [14C]glucosamine into individual glycosaminoglycans fractions under the conditions studied. The relative incorporation of radiolabeled precursor into heparan sulfate (and/or) heparin increased three- to fourfold. The relative incorporation of radiolabeled precursor remained constant for chondroitin 6-sulfate, whereas incorporation into chondroitin 4-sulfate and chondroitin (and/or) hyaluronic acid decreased. Under the conditions studied, retinoic acid did not appear to be cytotoxic and did exhibit selective control over glycosaminoglycan biosynthesis. It is suggested that the decreased incorporation of35SO4into glycosaminoglycans at hypervitaminosis A levels of retinol may be accounted for by the presence of low levels of retinoic acid, a naturally occurring metabolite.