Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence
Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence
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DOI：
10.1111/j.1751-7915.2011.00304.x
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发表时间：
2012-01-01
影响因子：
5.7
通讯作者：
Bertram, Ralph
中科院分区：
文献类型：
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作者：
Gauger, Tina;Weihs, Felix;Bertram, Ralph
描述了一种监测金黄色葡萄球菌蛋白质生产的细胞内方法。MCherry与十二肽末端融合后，能诱导四环素抑制因子(TetR)的表达。MCherry的产量依赖于时间和浓度，这可能与TetR控制的GFPmu2活性有关。这种方法有可能扩展到天然的金黄色葡萄球菌蛋白。
An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time- and concentration-dependent production of mCherry could be correlated to TetR-controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.